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dc.contributor.authorMathew, Asha
dc.contributor.authorCao, Hongliang
dc.contributor.authorCollin, Estelle
dc.contributor.authorWang, Wenxin
dc.contributor.authorPandit, Abhay
dc.date.accessioned2012-10-24T09:52:05Z
dc.date.available2012-10-24T09:52:05Z
dc.date.issued2012-06-02
dc.identifier.citationMathew, A,Cao, HL,Collin, E,Wang, WX,Pandit, A (2012) 'Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector'. International Journal Of Pharmaceutics, 434 :99-105.en_US
dc.identifier.issn0378-5173
dc.identifier.urihttp://hdl.handle.net/10379/3004
dc.description.abstractA unique hyperbranched polymeric system with a linear poly-2-dimethylaminoethyl methacrylate (pDMAEMA) block and a hyperbranched polyethylene glycol methyl ether methacrylate (PEGMEMA) and ethylene dimethacrylate (EGDMA) block was designed and synthesized via deactivation enhanced atom transfer radical polymerisation (DE-ATRP) for efficient gene delivery. Using this unique structure, with a linear pDMAEMA block, which efficiently binds to plasmid DNA (pDNA) and hyperbranched polyethylene glycol (PEG) based block as a protective shell, we were able to maintain high transfection levels without sacrificing cellular viability even at high doses. The transfection capability and cytotoxicity of the polymers over a range of pDNA concentration were analysed and the results were compared to commercially available transfection vectors such as polyethylene imine (branched PEI, 25 kDa), partially degraded poly(amido amine) dendrimer (dPAMAM; commercial name: SuperFect (R)) in fibroblasts and adipose tissue derived stem cells (ADSCs). (c) 2012 Elsevier B.V. All rights reserved.en_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal Of Pharmaceuticsen
dc.subject2-(Dimethylamino) ethyl methacrylateen_US
dc.subjectDeactivation enhanced atom transfer radical polymerisationen_US
dc.subjectGene therapyen_US
dc.titleHyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vectoren_US
dc.typeArticleen_US
dc.date.updated2012-10-18T16:26:34Z
dc.identifier.doiDOI 10.1016/j.ijpharm.2012.05.010
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid2208128
dc.local.contactAbhay Shashikant Pandit, Mechanical & Biomedical Eng, College Of Eng & Informatics, Room 304, Nfb Building, Ida Bus Park, Dangan, Nui Galway. 2758 Email: abhay.pandit@oegaillimh.ie
dc.local.copyrightcheckedNo
dc.local.versionPUBLISHED
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