dc.contributor.author | Mathew, Asha | |
dc.contributor.author | Cao, Hongliang | |
dc.contributor.author | Collin, Estelle | |
dc.contributor.author | Wang, Wenxin | |
dc.contributor.author | Pandit, Abhay | |
dc.date.accessioned | 2012-10-24T09:52:05Z | |
dc.date.available | 2012-10-24T09:52:05Z | |
dc.date.issued | 2012-06-02 | |
dc.identifier.citation | Mathew, A,Cao, HL,Collin, E,Wang, WX,Pandit, A (2012) 'Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector'. International Journal Of Pharmaceutics, 434 :99-105. | en_US |
dc.identifier.issn | 0378-5173 | |
dc.identifier.uri | http://hdl.handle.net/10379/3004 | |
dc.description.abstract | A unique hyperbranched polymeric system with a linear poly-2-dimethylaminoethyl methacrylate (pDMAEMA) block and a hyperbranched polyethylene glycol methyl ether methacrylate (PEGMEMA) and ethylene dimethacrylate (EGDMA) block was designed and synthesized via deactivation enhanced atom transfer radical polymerisation (DE-ATRP) for efficient gene delivery. Using this unique structure, with a linear pDMAEMA block, which efficiently binds to plasmid DNA (pDNA) and hyperbranched polyethylene glycol (PEG) based block as a protective shell, we were able to maintain high transfection levels without sacrificing cellular viability even at high doses. The transfection capability and cytotoxicity of the polymers over a range of pDNA concentration were analysed and the results were compared to commercially available transfection vectors such as polyethylene imine (branched PEI, 25 kDa), partially degraded poly(amido amine) dendrimer (dPAMAM; commercial name: SuperFect (R)) in fibroblasts and adipose tissue derived stem cells (ADSCs). (c) 2012 Elsevier B.V. All rights reserved. | en_US |
dc.format | application/pdf | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.relation.ispartof | International Journal Of Pharmaceutics | en |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | 2-(Dimethylamino) ethyl methacrylate | en_US |
dc.subject | Deactivation enhanced atom transfer radical polymerisation | en_US |
dc.subject | Gene therapy | en_US |
dc.title | Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector | en_US |
dc.type | Article | en_US |
dc.date.updated | 2012-10-18T16:26:34Z | |
dc.identifier.doi | DOI 10.1016/j.ijpharm.2012.05.010 | |
dc.description.peer-reviewed | peer-reviewed | |
dc.contributor.funder | |~| | |
dc.internal.rssid | 2208128 | |
dc.local.contact | Abhay Shashikant Pandit, Mechanical & Biomedical Eng, College Of Eng & Informatics, Room 304, Nfb Building, Ida Bus Park, Dangan, Nui Galway. 2758 Email: abhay.pandit@oegaillimh.ie | |
dc.local.copyrightchecked | No | |
dc.local.version | PUBLISHED | |
nui.item.downloads | 723 | |