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dc.contributor.authorLi, Boyan
dc.contributor.authorRyan, Paul W.
dc.contributor.authorShanahan, Michael
dc.contributor.authorRyder, Alan G.
dc.date.accessioned2012-08-23T11:15:45Z
dc.date.available2012-08-23T11:15:45Z
dc.date.issued2011-11
dc.identifier.citationB. Li, P.W. Ryan, M. Shanahan, K.J. Leister, and A.G. Ryder (2011) 'Fluorescence EEM Spectroscopy for Rapid Identification and Quality Evaluation of Cell Culture Media Components'. Applied Spectroscopy, 65 (11):1240-1249.en_US
dc.identifier.issn1943-3530
dc.identifier.urihttp://hdl.handle.net/10379/2941
dc.descriptionJournal article.en_US
dc.description.abstractThe application of fluorescence excitation-emission matrix (EEM) spectroscopy to the quantitative analysis of complex, aqueous solutions of cell culture media components was investigated.  These components, yeastolate, phytone, recombinant human insulin, eRDF basal medium, and four different chemically defined (CD) media, are used for the formulation of basal and feed media employed in the production of recombinant proteins using a Chinese Hamster Ovary (CHO) cell based process.  The comprehensive analysis (either identification or quality assessment) of these materials using chromatographic methods is time consuming and expensive, and not suitable for high throughput quality control.  The use of EEM in conjunction with multi-way chemometric methods provides for a rapid, non-destructive analytical method suitable for the screening of large numbers of samples.   Here we use multiway robust principal component analysis (MROBPCA) in conjunction with N-way partial least-squares-discriminant analysis (NPLS-DA) to develop a robust routine for both the identification and quality evaluation of these important cell culture materials:  These methods are applicable to a wide range of complex mixtures since they do not rely on any predetermined compositional or property information, thus making them potentially very useful for sample handling, tracking, and quality assessment in biopharmaceutical industries. en_US
dc.description.sponsorshipIndustrial Development Authority; Bristol-Myers Squibb [Centre for BioAnalytical Sciences]en_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.publisherIngentaen_US
dc.relation.ispartofApplied Spectroscopyen
dc.subjectFluorescence,en_US
dc.subjectEEM,en_US
dc.subjectCell culture media,en_US
dc.subjectChemometricsen_US
dc.titleFluorescence EEM Spectroscopy for Rapid Identification and Quality Evaluation of Cell Culture Media Components.en_US
dc.typeArticleen_US
dc.date.updated2012-08-17T14:16:26Z
dc.identifier.doihttp://dx.doi.org/10.1366/11-06383
dc.local.publishedsourcehttp://dx.doi.org/10.1366/11-06383en_US
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|Industrial Contracts|~|
dc.internal.rssid1284880
dc.local.contactAlan Ryder, School Of Chemistry, Room 228, Arts/Science Building, Nui Galway. 2943 Email: alan.ryder@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
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