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dc.contributor.authorRyan, Paul W.
dc.contributor.authorLi, Boyan
dc.contributor.authorShanahan, Michael
dc.contributor.authorRyder, Alan G.
dc.date.accessioned2012-08-22T11:03:54Z
dc.date.available2012-08-22T11:03:54Z
dc.date.issued2010
dc.identifier.citationRyan, P.W., Li, B., Shanahan, M., Leister, K.J., Ryder ,A.G. (2010) 'Prediction of Cell Culture Media performance using Fluorescence Spectroscopy'. Analytical Chemistry, 82 :1311-1317.en_US
dc.identifier.issn1520-6882
dc.identifier.urihttp://www.nuigalway.ie/nanoscale/publications.html
dc.identifier.urihttp://hdl.handle.net/10379/2932
dc.descriptionJournal articleen_US
dc.description.abstractCell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in efficient manufacturing. We are exploring the use of rapid holistic analytical methods that can be used for routine screening of cell culture media used in industrial biotechnology.  The application of rapid fluorescence spectroscopic techniques to the routine analysis of cell culture media (Chinese hamster ovary cell-based manufacture) was investigated. We have developed robust methods which can be used to identify compositional changes and ultimately predict the efficacy of individual fed batchmedia in terms of downstream protein product yield with an accuracy of (0.13 g/L. This is achieved through the implementation of chemometric methods such as multiway robust principal component analysis (MROBPCA), and n-way partial least-squares-discriminant analysis and regression (NPLS-DA and NPLS). This ability to observe compositional changes and predict product yield before media use has enormous potential and should permit the effective elimination of one of the major process variables leading to more consistent product quality and improved yield. These robust and reliable methods have the potential to become an important part of upstream biopharmaceutical quality control and analysis.en_US
dc.description.sponsorshipIrish Industrial Development Authority; Bristol-Myers Squibb;en_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.publisherACS Publicationsen_US
dc.relation.ispartofAnalytical Chemistryen
dc.subjectBiopharmaceuticalen_US
dc.subjectFluorescenceen_US
dc.subjectChemometricsen_US
dc.subjectquantitativeen_US
dc.subjectproteinen_US
dc.titlePrediction of Cell Culture Media performance using Fluorescence Spectroscopyen_US
dc.typeArticleen_US
dc.date.updated2012-08-17T14:00:25Z
dc.identifier.doihttp://dx.doi.org/10.1021/ac902337c
dc.local.publishedsourcehttp://dx.doi.org/10.1021/ac902337cen_US
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|Other|~|
dc.internal.rssid1158820
dc.local.contactAlan Ryder, School Of Chemistry, Room 228, Arts/Science Building, Nui Galway. 2943 Email: alan.ryder@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
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