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dc.contributor.authorRyder, Alan G.
dc.contributor.authorPower, Sarah
dc.contributor.authorGlynn, Thomas J.
dc.date.accessioned2012-08-22T09:59:43Z
dc.date.available2012-08-22T09:59:43Z
dc.date.issued2003
dc.identifier.citationRyder, AG,Power, S,Glynn, TJ (2003) 'Evaluation of acridine in Nafion as a fluorescence-lifetime-based pH sensor'. Applied Spectroscopy, 57 (1):73-79.en_US
dc.identifier.issn1943-3530
dc.identifier.urihttp://www.opticsinfobase.org/as/abstract.cfm?URI=as-57-1-73
dc.identifier.urihttp://hdl.handle.net/10379/2928
dc.descriptionJournal articleen_US
dc.description.abstractWe report a novel fluorescence-lifetime-based pH sensing method that utilizes acridine incorporated into Nation (AcNaf) as the fluorescent indicator. The AcNaf sensor is excited using a 380 nm light emitting diode (LED) and the fluorescence lifetimes are measured at 450 and 500 nm. The fluorescence behavior of acridine as a function of pH in aqueous phosphate buffers and incorporated into the Nation membrane has been investigated. The results show that incorporating acridine into Nation changes the apparent ground-state pK(a) from similar to5.45 to similar to9, while the apparent excited-state pK(a)* is only slightly changed (similar to9.4 in 0.1 M phosphate buffer). The AcNaf film shows a good pH response with a change in average lifetime of similar to19 ns (at an emission wavelength of 450 nm) over the pH 8 to 10 range. We also show that excited-state protonation does not occur in the AcNaf sensor film and that chloride quenching cannot occur because of the permselective nature of Nafion. We also discuss how the unique structure of Nation affects the fluorescence behavior of acridine at various pH values and examine the impact of buffer concentration on apparent pK(a) and pH sensing ability.en_US
dc.description.sponsorshipNUI Galway Millennium Research Grant; Enterprise Ireland Research Innovation Fund (IF/2001/061); HEA Programme for Researchen_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.relation.ispartofApplied Spectroscopyen
dc.subjectFluorescence lifetimeen_US
dc.subjectAcridineen_US
dc.subjectSensoren_US
dc.subjectNafionen_US
dc.subjectUltravioleten_US
dc.subjectLight emitting diodeen_US
dc.subjectpHen_US
dc.subjectTime-resolved fluorescenceen_US
dc.subjectLight-emitting diodeen_US
dc.subjectPhase-modulation fluorometryen_US
dc.subjectOptical sensorsen_US
dc.subjectSilica-gelen_US
dc.subjectDecay timeen_US
dc.subjectNanoseconden_US
dc.subjectLuminescenceen_US
dc.subjectStateen_US
dc.subjectAbsorptionen_US
dc.titleEvaluation of acridine in Nafion as a fluorescence-lifetime-based pH sensoren_US
dc.typeArticleen_US
dc.date.updated2012-08-17T12:55:16Z
dc.local.publishedsourcehttp://www.opticsinfobase.org/as/abstract.cfm?URI=as-57-1-73en_US
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid1346951
dc.local.contactAlan Ryder, School Of Chemistry, Room 228, Arts/Science Building, Nui Galway. 2943 Email: alan.ryder@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
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