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dc.contributor.authorGupta, Sanjeev
dc.contributor.authorRead, D.E.
dc.contributor.authorDeepti, A.
dc.contributor.authorCawley, K.
dc.contributor.authorGupta, A.
dc.contributor.authorOommen, D.
dc.contributor.authorSamal, Afshin
dc.date.accessioned2012-07-09T10:01:38Z
dc.date.available2012-07-09T10:01:38Z
dc.date.issued2012
dc.identifier.citationS Gupta, DE Read, A Deepti, K Cawley, A Gupta, D Oommen, T Verfaillie4, S Matus, MA Smith, JL Mott, (2012) 'Perk-dependent repression of miR-106b-25 cluster is required for ER stress-induced apoptosis'. Cell Death & Disease, .en_US
dc.identifier.urihttp://hdl.handle.net/10379/2901
dc.description.abstractActivation of the unfolded protein response sensor PKR-like endoplasmic reticulum kinase (Perk) attenuates endoplasmic reticulum (ER) stress levels. Conversantly, if the damage is too severe and ER function cannot be restored, this signaling branch triggers apoptosis. Bcl-2 homology 3-only family member Bim is essential for ER stress-induced apoptosis. However, the regulatory mechanisms controlling Bim activation under ER stress conditions are not well understood. Here, we show that downregulation of the miR-106b-25 cluster contributes to ER stress-induced apoptosis and the upregulation of Bim. Hypericin-mediated photo-oxidative ER damage induced Perk-dependent cell death and led to a significant decrease in the levels of miRNAs belonging to miR-106b-25 cluster in wild-type (WT) but not in Perk-/- MEFs. Further, we show that expression of miR-106b-25 and Mcm-7 (host gene of miR-106b-25) is co-regulated through the transcription factors Atf4 (activating transcription factor 4) and Nrf2 (nuclear factor-erythroid-2-related factor 2). ER stress increased the activity of WT Bim 3-UTR (untranslated region) construct but not the miR-106b-25 recognition site-mutated Bim 3-UTR construct. Overexpression of miR-106b-25 cluster inhibits ER stress-induced cell death in WT but did not confer any further protection in Bim-knockdown cells. Further, we show downregulation in the levels of miR-106b-25 cluster in the symptomatic SOD1G86R transgenic mice. Our results suggest a molecular mechanism whereby repression of miR-106b-25 cluster has an important role in ER stress-mediated increase in Bim and apoptosis.en_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.relation.ispartofCell Death & Diseaseen
dc.subjectmiR-106b-25en_US
dc.subjectUnfolded protein responseen_US
dc.subjectApoptosisen_US
dc.subjectBimen_US
dc.subjectATF4en_US
dc.subjectNRF2en_US
dc.titlePerk-dependent repression of miR-106b-25 cluster is required for ER stress-induced apoptosisen_US
dc.typeArticleen_US
dc.date.updated2012-07-03T10:56:05Z
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderSFI
dc.contributor.funderHRB
dc.internal.rssid1906704
dc.local.contactSanjeev Gupta, Pathology, School Of Medicine, Nui Galway. 544488 Email: sanjeev.gupta@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
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