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dc.contributor.authorDonoghue, Mark T. A.
dc.contributor.authorSpillane, Charles
dc.date.accessioned2012-04-30T17:13:52Z
dc.date.available2012-04-30T17:13:52Z
dc.date.issued2011
dc.identifier.citationWolff, P,Weinhofer, I,Seguin, J,Roszak, P,Beisel, C,Donoghue, MTA,Spillane, C,Nordborg, M,Rehmsmeier, M,Kohler, C (2011) High-resolution analysis of parent-of-origin allelic expression in the Arabidopsis endosperm. Plos Genetics, 7 .en_US
dc.identifier.urihttp://hdl.handle.net/10379/2706
dc.description.abstractGenomic imprinting is an epigenetic phenomenon leading to parent-of-origin specific differential expression of maternally and paternally inherited alleles. In plants, genomic imprinting has mainly been observed in the endosperm, an ephemeral triploid tissue derived after fertilization of the diploid central cell with a haploid sperm cell. In an effort to identify novel imprinted genes in Arabidopsis thaliana, we generated deep sequencing RNA profiles of F1 hybrid seeds derived after reciprocal crosses of Arabidopsis Col-0 and Bur-0 accessions. Using polymorphic sites to quantify allele-specific expression levels, we could identify more than 60 genes with potential parent-of-origin specific expression. By analyzing the distribution of DNA methylation and epigenetic marks established by Polycomb group (PcG) proteins using publicly available datasets, we suggest that for maternally expressed genes (MEGs) repression of the paternally inherited alleles largely depends on DNA methylation or PcG-mediated repression, whereas repression of the maternal alleles of paternally expressed genes (PEGs) predominantly depends on PcG proteins. While maternal alleles of MEGs are also targeted by PcG proteins, such targeting does not cause complete repression. Candidate MEGs and PEGs are enriched for cis-proximal transposons, suggesting that transposons might be a driving force for the evolution of imprinted genes in Arabidopsis. In addition, we find that MEGs and PEGs are significantly faster evolving when compared to other genes in the genome. In contrast to the predominant location of mammalian imprinted genes in clusters, cluster formation was only detected for few MEGs and PEGs, suggesting that clustering is not a major requirement for imprinted gene regulation in Arabidopsis.en_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.relation.ispartofPlos Geneticsen
dc.subjectDNA METHYLATIONen_US
dc.subjectSEED DEVELOPMENTen_US
dc.subjectPOLYCOMB GENEen_US
dc.subjectTRANSPOSABLE ELEMENTSen_US
dc.subjectTRANSCRIPTION FACTORSen_US
dc.subjectFEMALE GAMETOPHYTEen_US
dc.subjectTANDEM REPEATSen_US
dc.subjectDOMAIN PROTEINen_US
dc.subjectIMPRINTED GENEen_US
dc.subjectMEDEA LOCUSen_US
dc.titleHigh-resolution analysis of parent-of-origin allelic expression in the Arabidopsis endosperm.en_US
dc.typeArticleen_US
dc.date.updated2012-04-30T17:08:06Z
dc.identifier.doiARTN e1002126
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid1051796
dc.local.contactCharles Spillane, Botany, School Of Natural Sciences, Nui Galway. Email: charles.spillane@nuigalway.ie
dc.local.copyrightcheckedYes
dc.local.versionACCEPTED
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