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dc.contributor.authorButler, Ryan K.
dc.contributor.authorFord, Gemma K.
dc.contributor.authorHogan, Michelle
dc.contributor.authorRoche, Michelle
dc.contributor.authorDoyle, Karen M.
dc.contributor.authorKelly, John P.
dc.contributor.authorFinn, David P.
dc.date.accessioned2012-01-20T10:54:16Z
dc.date.available2012-11-21T15:57:07Z
dc.date.issued2011-09-17
dc.identifier.citationButler RK, Ford GK, Hogan M, Roche M, Doyle KM, Kelly JP, Kendall DA, Chapman V, Finn DP (2011). Fear-induced suppression of nociceptive behaviour and activation of signal transduction molecules in the rat periaqueductal grey: role of fatty acid amide hydrolase.en_US
dc.identifier.urihttp://hdl.handle.net/10379/2497
dc.description.abstractThe endocannabinoid system regulates nociception and aversion and mediates fear-conditioned analgesia (FCA). We investigated the effects of the fatty acid amide hydrolase (FAAH) inhibitor URB597, which inhibits the catabolism of the endocannabinoid anandamide and related N-acylethanolamines, on expression of FCA and fear and pain related behaviour per se in rats. We also examined associated alterations in the expression of the signal transduction molecule phospho-Akt in the periaqueductal grey (PAG) by immunoblotting. FCA was modelled by assessing formalin-evoked nociceptive behaviour in an arena previously paired with footshock. URB597 (0.3 mg/kg, i.p.) enhanced FCA and increased fear-related behaviour in formalin-treated rats. Conditioned fear per se in non-formalin-treated rats was associated with increased expression of phospho-Akt in the PAG. URB597 reduced the expression of fear-related behaviour in the early part of the trial, an effect that was accompanied by attenuation of the fear-induced increase in phospho-Akt expression in the PAG. Intra-plantar injection of formalin also reduced the fear-induced increase in phospho-Akt expression. These data provide evidence for a role of FAAH in FCA, fear responding in the presence or absence of nociceptive tone, and fear-evoked increases in PAG phospho-Akt expression. In addition, the results suggest that fear-evoked activation of Akt signalling in the PAG is abolished in the presence of nociceptive tone.en_US
dc.formatapplication/pdfen_US
dc.language.isoenen_US
dc.publisherSageen_US
dc.subjectAkt (protein kinase B)en_US
dc.subjectEndocannabinoidsen_US
dc.subjectFatty acid amide hydrolase (FAAH)en_US
dc.subjectFearen_US
dc.subjectPainen_US
dc.subjectPeriaqueductal grey (PAG)en_US
dc.subjectRatsen_US
dc.subjectPharmacology &Therapeuticsen_US
dc.titleFear-induced suppression of nociceptive behaviour and activation of signal transduction molecules in the rat periaqueductal grey: role of fatty acid amide hydrolase.en_US
dc.typeArticleen_US
dc.local.publishedsourcehttp://dx.doi.org/10.1177/0269881111413823en_US
dc.description.peer-reviewedpeer-revieweden_US
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