Browsing National Centre for Biomedical Engineering Science by Author "Gerlach, Jared Q."
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Campylobacter jejuni strain discrimination and temperature-dependent glycome expression profiling by lectin microarray
Kilcoyne, Michelle; Twomey, Marcus E.; Gerlach, Jared Q.; Kane, Marian; Moran, Anthony P.; Joshi, Lokesh (Elsevier, 2014-02-12)Gram-negative Campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans worldwide and the most frequently identified infectious trigger in patients developing Guillain-Barré syndrome (GBS). While ... -
Construction of a Natural Mucin Microarray and Interrogation for Biologically Relevant Glyco-Epitopes
Kilcoyne, Michelle; Gerlach, Jared Q.; Kane, Marian; Joshi, Lokesh (2012)Mucins are the principal components of mucus, and mucin glycosylation has important roles in defense, microbial adhesion, immunomodulation, inflammation, and cancer. Mucin expression and glycosylation are dynamic, responding ... -
Differential release of high mannose structural isoforms by fungal and bacterial endo-β-N-acetylglucosaminidases
Gerlach, Jared Q.; Kilcoyne, Michelle; Farrell, Mark P.; Kane, Marian; Joshi, Lokesh (Royal Society of Chemistry, 2012-02-28)Endo-beta-N-acetylglucosaminidases (ENGases) are widely used to remove N-linked oligosaccharides from glycoproteins for glycomic and proteomic studies and biopharmaceutical processes. Although several ENGases are widely ... -
Periodic acid-Schiff's reagent assay for carbohydrates in a microtiter plate format
Kilcoyle, Michelle; Gerlach, Jared Q.; Farrell, Mark P.; Joshi, Lokesh (2011)Microtiter plate colorimetric assays are widely used for analysis of carbohydrates and glycoconjugates. However, mucins are often not easily detected, as they have low neutral sugar content. We have adapted and optimised ... -
Surface chemistry and linker effects on lectin-carbohydrate recognition for glycan microarrays
Kilcoyne, Michelle; Gerlach, Jared Q.; Kane, Marian; Joshi, Lokesh (2012)Glycan microarrays are an increasingly utilised tool for analysis of protein-carbohydrate interactions and a variety of glycan-containing molecules and slide chemistries have been used to array carbohydrates on microarray ...