Browsing Research Institutes and Centres by Author "Joshi, Lokesh"
Now showing items 1-8 of 8
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Campylobacter jejuni strain discrimination and temperature-dependent glycome expression profiling by lectin microarray
Kilcoyne, Michelle; Twomey, Marcus E.; Gerlach, Jared Q.; Kane, Marian; Moran, Anthony P.; Joshi, Lokesh (Elsevier, 2014-02-12)Gram-negative Campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans worldwide and the most frequently identified infectious trigger in patients developing Guillain-Barré syndrome (GBS). While ... -
Construction of a Natural Mucin Microarray and Interrogation for Biologically Relevant Glyco-Epitopes
Kilcoyne, Michelle; Gerlach, Jared Q.; Kane, Marian; Joshi, Lokesh (2012)Mucins are the principal components of mucus, and mucin glycosylation has important roles in defense, microbial adhesion, immunomodulation, inflammation, and cancer. Mucin expression and glycosylation are dynamic, responding ... -
Differential release of high mannose structural isoforms by fungal and bacterial endo-β-N-acetylglucosaminidases
Gerlach, Jared Q.; Kilcoyne, Michelle; Farrell, Mark P.; Kane, Marian; Joshi, Lokesh (Royal Society of Chemistry, 2012-02-28)Endo-beta-N-acetylglucosaminidases (ENGases) are widely used to remove N-linked oligosaccharides from glycoproteins for glycomic and proteomic studies and biopharmaceutical processes. Although several ENGases are widely ... -
Donor bone marrow-derived dendritic cells prolong corneal allograft survival and promote an intragraft immunoregulatory milieu.
O'Flynn, Lisa; Treacy, Oliver; Ryan, Aideen E.; Morcos, Maurice; Cregg, Marese; Gerlach, Jared; Joshi, Lokesh; Nosov, Mikhail; Ritter, Thomas (Nature Publishing Group, 2013-08)Investigations into cell therapies for application in organ transplantation have grown. Here, we describe the ex vivo generation of donor bone marrow-derived dendritic cells (BMDCs) and glucocorticoid-treated BMDCs with ... -
Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies.
Kumar, Pramod; Satyam, Abhigyan; Fan, Xingliang; Collin, Estelle; Rochev, Yury; Joshi, Lokesh; Pandit, Abhay; Zeugolis, Dimitrios I. (Nature Publishing Group, 2015-03-04)Therapeutic strategies based on the principles of tissue engineering by self-assembly put forward the notion that functional regeneration can be achieved by utilising the inherent capacity of cells to create highly ... -
Neuronal glycosylation differentials in normal, injured and chondroitinase-treated environments
Kilcoyne, Michelle; Sharma, Shashank; McDevitt, Niamh; O'Leary, Claire; Joshi, Lokesh; McMahon, Siobhán S. (2012)Glycosylation is found ubiquitously throughout the central nervous system (CNS). Chondroitin sulphate proteoglycans (CSPGs) are a group of molecules heavily substituted with glycosaminoglycans (GAGs) and are found in the ... -
Periodic acid-Schiff's reagent assay for carbohydrates in a microtiter plate format
Kilcoyle, Michelle; Gerlach, Jared Q.; Farrell, Mark P.; Joshi, Lokesh (2011)Microtiter plate colorimetric assays are widely used for analysis of carbohydrates and glycoconjugates. However, mucins are often not easily detected, as they have low neutral sugar content. We have adapted and optimised ... -
Surface chemistry and linker effects on lectin-carbohydrate recognition for glycan microarrays
Kilcoyne, Michelle; Gerlach, Jared Q.; Kane, Marian; Joshi, Lokesh (2012)Glycan microarrays are an increasingly utilised tool for analysis of protein-carbohydrate interactions and a variety of glycan-containing molecules and slide chemistries have been used to array carbohydrates on microarray ...