Browsing University of Galway Theses by Author "Santocanale, Corrado"
Now showing items 1-11 of 11
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CDC7 kinase promotes replication DNA double-strand breaks upon replication fork stalling and its inhibition causes an inflammatory response and senescence
Cazzaniga, Chiara (NUI Galway, 2022-12-08)CDC7 is an essential serine-threonine kinase that has an important role in the initiation of DNA replication. CDC7 phosphorylates several sites of the MCM helicase complex allowing recruitment of CDC45, among other factors, ... -
CDC7 kinase regulates DNA replication fork processing and its inhibition is modulated by PTBP1
Quinlan, Aisling (NUI Galway, 2022-09-21)CDC7 is an essential serine/threonine kinase that regulates the initiation of DNA replication by phosphorylating several sites of the MCM helicase complex. Several inhibitors of CDC7 are available that reduce cell proliferation ... -
Characterisation of the cellular responses to CDC7 inhibition in breast-derived human cells
Quach Thi Thu, Huong (2017-10-06)CDC7 plays a role in DNA replication initiation and cell cycle regulation. Inhibition of CDC7 kinase by siRNA triggers a p53-independent apoptotic cell death in cancer cells but not in human fibroblast suggesting this ... -
DNA mediated chromatin pull-down: a novel method for the study of chromatin replication
Kliszczak, Anna Ewelina (2012-04-27)Chromatin replication involves duplicating DNA while maintaining epigenetic information. These processes are critical for genome stability and for preserving cell-type identity. To investigate how these processes are ... -
Examining a new class of dual CDC7/CDK9 inhibitors, mechanisms of proliferation, and proliferation-based stratification in myeloma
Coyne, Mark Robert (2014-09-05)A key feature of progressive myeloma is a relative increase in proliferation. The DBF4-dependent kinase (DDK), cell division cycle 7 (CDC7), is an essential kinase for initiation of DNA replication. This thesis assesses ... -
Identification of IRS4 and TOP2A as novel CDC7 kinase interacting proteins
Wu, Kevin Zhi Loong (2016-02-08)CDC7 is an essential serine/threonine kinase required for the initiation of DNA replication in eukaryotic cells, through phosphorylating the MCM helicase complex. Formation of an active kinase requires the binding to ... -
Investigating the roles of Chl1 helicase in DNA damage tolerance pathways
Reyes, Teresa Anne Clarisse (NUI Galway, 2023-01-09)Faithful DNA replication is fundamental to ensure proper genomic stability. Eukaryotic cells have evolved sophisticated networks to deal with DNA lesions encountered during chromosome replication. The DNA damage response ... -
Life with crippled microtubules: How yeast and human cells deal with forced microtubule depolymerization
Pavani, Mattia (NUI Galway, 2023-01-25)Microtubule poisons are microtubule targeting agents widely used in cancer treatment. These drugs are given to cancer patients in cycles, and resistant cells can emerge both after the first or the following rounds of ... -
Maintenance of the spindle assembly checkpoint by PLK1 and CDC7 kinases and characterisation of cell lines carrying mutations in the genes coding for the CDC7 regulatory subunits
O'Connor, Aisling (2016-11-15)The Spindle Assembly Checkpoint (SAC) ensures that accurate chromosome segregation occurs, by preventing progression into anaphase until all kinetochore-microtubule attachments are stable and bi-polar. During a mitotic ... -
A role for USP9X in homologous recombination repair of DNA double strand breaks
O'Dea, Rachel (NUI Galway, 2020-02-11)In order to prevent the deleterious effects of a variety of genotoxic agents, cells have developed complex surveillance mechanisms and multiple DNA repair pathways that allow them to maintain genome integrity. The ... -
USP9X controls DNA replication fork stability and the replication stress checkpoint through Claspin
McGarry, Edel (2016-02-05)Cells possess checkpoint pathways which are important for maintaining genome stability and preventing cancer. These signalling pathways include the ATR and CHK1 kinases which are activated in response to DNA damage or ...