Browsing College of Science by Author "Ryder, Alan G."
Now showing items 1-20 of 66
-
Accurate anisotropy recovery from fluorophore mixtures using Multivariate Curve Resolution (MCR)
Casamayou-Boucau, Yannick; Ryder, Alan G. (Elsevier, 2017-11-22)Anisotropy resolved multidimensional emission spectroscopy (ARMES) provides valuable insights into multi-fluorophore systems like proteins that have complex overlapping emission bands. The method combines multidimensional ... -
Analysis of crude petroleum oils using fluorescence spectroscopy.
Ryder, Alan G. (Springer, 2005)Crude oil is defined as a mixture of hydrocarbons that existed in the liquid phase in natural underground reservoirs and remains liquid at atmospheric pressure after passing through surface separating facilities (joint ... -
Analysis of hydrocarbon bearing fluid inclusions HCFI) using time-resolved fluorescence spectroscopy
Przyjalgowski, Milosz A.; Ryder, Alan G.; Feely, Martin; Glynn, Thomas J. (Society of Photo-optical Instrumentation Engineers, 2005)Hydrocarbon-bearing fluid inclusions (HCFI) are microscopic cavities within rocks that are filled with petroleum oil, the composition of which may not have changed since the trapping event. Thus, the composition of that ... -
Analyzing protein conjugation reactions for antibody-drug conjugate synthesis using polarized excitation emission matrix spectroscopy
de Faria e Silva, Ana L.; Ryder, Alan G. (Wiley, 2022-09-07)Antibody-drug conjugates (ADCs) are promising anticancer therapeutics, which offer important advantages compared to more classical therapies. There are a variety of ADC critical quality attributes (CQAs) such as the protein ... -
Anisotropy resolved multidimensional emission spectroscopy (ARMES): A new tool for protein analysis
Groza, Radu Constantin; Ryder, Alan G.; Li, Boyan (Elsevier, 2015-07-30)Structural analysis of proteins using the emission of intrinsic fluorophores is complicated by spectral overlap. Anisotropy resolved multidimensional emission spectroscopy (ARMES) overcame the overlap problem by the use ... -
Applications of raman spectroscopy in biopharmaceutical manufacturing: A short review
Buckley, Kevin; Ryder, Alan G. (Sage Publications, 2017-05-23)The production of active pharmaceutical ingredients (APIs) is currently undergoing its biggest transformation in a century. The changes are based on the rapid and dramatic introduction of protein- and macromolecule-based ... -
Calibration, standardization, and quantitative analysis of multidimensional fluorescence (MDF) measurements on complex mixtures (IUPAC Technical Report)
Ryder, Alan G.; Stedmon, Colin A.; Harrit, Niels; Bro, Rasmus (De Gruyter, 2017-11-15)This IUPAC Technical Report describes and compares the currently applied methods for the calibration and standardization of multi-dimensional fluorescence (MDF) spectroscopy data as well as recommendations on the correct ... -
Cell culture media analysis using rapid spectroscopic methods
Ryder, Alan G. (Elsevier, 2018-09-24)Cell culture media (CCM) used in industrial biotechnology are complex mixtures of molecules and elements that are inherently difficult to analyze comprehensively. CCM quality analysis is of utmost importance for efficient ... -
Cell cycle-dependent mobility of Cdc45 determined in vivo by fluorescence correlation spectroscopy
Broderick, Ronan; Ramadurai, Sivaramakrishnan; Tóth, Katalin; Togashi, Denisio M.; Ryder, Alan G.; Langowski, Jörg; Nasheuer, Heinz-Peter (Public Library of Science, 2012-04-19)Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS) in vivo in asynchronous ... -
Cell cycle-dependent mobility of Cdc45 in living cells determined by fluorescence correlation spectroscopy.
Broderick, Ronan; Ramadurai, Sivaramakrishnan; Togashi, Denisio M.; Ryder, Alan G.; Langowski, Jorg (Public Library of Science, 2012)Eukaryotic DNA replication is a dynamic process requiring the co-operation of specific replication proteins. We measured the mobility of eGFP-Cdc45 by Fluorescence Correlation Spectroscopy (FCS) in vivo in asynchronous ... -
Characterization of Lysozyme PEGylation products using polarized Excitation Emission Matrix (pEEM) spectroscopy
de Faria E Silva, Ana Luiza; Elcoroaristizabal, Saioa; Ryder, Alan G. (Wiley, 2020-07-07)The growing use of therapeutic proteins requires accurate measurement techniques for measuring biophysical and structural changes during manufacturing. This is particularly true for PEGylation of proteins, because ... -
Chemometric approaches to low-content quantification (LCQ) in solid-state mixtures using Raman mapping spectroscopy.
Li, Boyan; Casamayou-Boucau, Yannick; Calvet, Amandine; Ryder, Alan G. (Royal Society of Chemistry, 2017-10-30)The low-content quantification (LCQ) of active pharmaceutical ingredients or impurities in solid mixtures is important in pharmaceutical manufacturing and analysis. We previously demonstrated the feasibility of using Raman ... -
Classification of narcotics in solid mixtures using principal component analysis and Raman spectroscopy
Ryder, Alan G. (ASTM International, 2002-03-01)Eighty-five solid samples consisting of illegal narcotics diluted with several different materials were analyzed by near-infrared (785 nm excitation) Raman spectroscopy. Principal Component Analysis (PCA) was employed to ... -
Comparison of the fluorescence behavior of a biocrude oil and crude petroleum oils
Ryder, Alan G. (ACS Publications (American Chemical Society), 2006-02-15)The production and characterization of biocrude and petroleum like products from natural, renewable resources such as oil-bearing plant seeds is an interesting area of research at the moment. The present article discusses ... -
Comprehensive, quantitative bioprocess productivity monitoring using fluorescence EEM spectroscopy and chemometrics
Calvet, Amandine; Ryder, Alan G. (Royal Society of Chemistry, 2014)This study demonstrates the application of fluorescence excitation-emission matrix (EEM) spectroscopy to the quantitative predictive analysis of recombinant glycoprotein production cultured in a Chinese hamster ovary (CHO) ... -
Development of a rapid Polarized Total Synchronous Fluorescence Spectroscopy (pTSFS) method for protein quantification in a model bioreactor broth
Boateng, Bernard O.; Elcoroaristizabal, Saioa; Ryder, Alan G. (Wiley, 2021-01-27)Protein quantification during bioprocess monitoring is essential for biopharmaceutical manufacturing and is complicated by the complex chemical composition of the bioreactor broth. Here we present the early-stage development ... -
Evaluating the interaction of human serum albumin (HSA) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes in different aqueous environments using anisotropy resolved multi-dimensional emission spectroscopy (ARMES)
Gordon, Fiona; Casamayou-Boucau, Yannick; Ryder, Alan G. (Elsevier, 2022-01-07)Studying the interaction between plasma proteins and liposomes is critical, particularly for their use as drug delivery systems. Here, the efficacy of anisotropy resolved multidimensional emission spectroscopy (ARMES) for ... -
Evaluation of acridine in Nafion as a fluorescence-lifetime-based pH sensor
Ryder, Alan G.; Power, Sarah; Glynn, Thomas J. (2003)We report a novel fluorescence-lifetime-based pH sensing method that utilizes acridine incorporated into Nation (AcNaf) as the fluorescent indicator. The AcNaf sensor is excited using a 380 nm light emitting diode (LED) ... -
An excitation emission fluorescence lifetime spectrometer using a frequency doubled supercontinuum laser source
Melnikau, Dzmitry; Elcoroaristizabal, Saioa; Ryder, Alan G. (IOP Publishing, 2018-09-03)The accurate fluorescence analysis of complex, multi-fluorophore containing proteins requires the use of multi-dimensional measurement techniques. For the measurement of intrinsic fluorescence from tyrosine (Tyr) and ... -
Extended wavelength anisotropy resolved multidimensional emission spectroscopy (ARMES) measurements: better filters, validation standards, and Rayleigh scatter removal methods
Casamayou-Boucau, Yannick; Ryder, Alan G. (IOP Publishing, 2017-08-17)Anisotropy resolved multidimensional emission spectroscopy (ARMES) provides valuable insights into multi-fluorophore proteins (Groza et al 2015 Anal. Chim. Acta 886 133-42). Fluorescence anisotropy adds to the multidimensional ...