Browsing College of Science by Author "Li, Boyan"
Now showing items 1-13 of 13
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Anisotropy resolved multidimensional emission spectroscopy (ARMES): A new tool for protein analysis
Groza, Radu Constantin; Ryder, Alan G.; Li, Boyan (Elsevier, 2015-07-30)Structural analysis of proteins using the emission of intrinsic fluorophores is complicated by spectral overlap. Anisotropy resolved multidimensional emission spectroscopy (ARMES) overcame the overlap problem by the use ... -
Chemometric approaches to low-content quantification (LCQ) in solid-state mixtures using Raman mapping spectroscopy.
Li, Boyan; Casamayou-Boucau, Yannick; Calvet, Amandine; Ryder, Alan G. (Royal Society of Chemistry, 2017-10-30)The low-content quantification (LCQ) of active pharmaceutical ingredients or impurities in solid mixtures is important in pharmaceutical manufacturing and analysis. We previously demonstrated the feasibility of using Raman ... -
Fluorescence EEM Spectroscopy for Rapid Identification and Quality Evaluation of Cell Culture Media Components.
Li, Boyan; Ryan, Paul W.; Shanahan, Michael; Ryder, Alan G. (Ingenta, 2011-11)The application of fluorescence excitation-emission matrix (EEM) spectroscopy to the quantitative analysis of complex, aqueous solutions of cell culture media components was investigated. These components, yeastolate, ... -
Kernel principal component analysis residual diagnosis (KPCARD): an automated method to remove cosmic ray artefacts in Raman spectra.
Li, Boyan; Calvet, Amandine; Casamayou-Boucau, Yannick; Ryder, Alan G. (2016)A new, fully automated, rapid method, referred to as kernel principal component analysis residual diagnosis (KPCARD), is proposed for removing cosmic ray artifacts (CRAs) in Raman spectra, and in particular for large Raman ... -
Low-content quantification in powders using Raman spectroscopy: a facile chemometric approach to sub 0.1% limits of detection.
Li, Boyan; Calvet, Amandine; Ryder, Alan G.; Casamayou-Boucau, Yannick; Morris, Cheryl (American Chemical Society, 2015-02-23)A robust and accurate analytical methodology for low-content (<0.1%) quantification in the solid-state using Raman spectroscopy, sub-sampling, and chemometrics was demonstrated using a piracetam–proline model. The method ... -
Performance Monitoring of a Mammalian Cell Based Bioprocess using Raman spectroscopy.
Ryder, Alan G.; Li, Boyan; Ray, Bryan H. (2013)Being able to predict the final product yield at all stages in long-running, industrial, mammalian cell culture processes is vital for both operational efficiency, process consistency, and the implementation of Quality by ... -
Prediction of Cell Culture Media performance using Fluorescence Spectroscopy
Ryan, Paul W.; Li, Boyan; Shanahan, Michael; Ryder, Alan G. (ACS Publications, 2010)Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in ... -
Prediction of cell culture media performance using fluorescence spectroscopy.
Ryan, Paul W.; Li, Boyan; Shanahan, Michael; Ryder, Alan G. (American Chemical Society, 2010)Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in ... -
Rapid characterization and quality control of complex cell culture media solutions using Raman spectroscopy and chemometrics
Li, Boyan; Ryan, Paul W.; Ray, Bryan H.; Sirimuthu, Narayana M. S.; Ryder, Alan G. (2010)The use of Raman spectroscopy coupled with chemometrics for the rapid identification, characterization, and quality assessment of Complex cell culture media components used for industrial mammalian cell culture was ... -
Rapid quantification of tryptophan and tyrosine in chemically defined cell culture media using fluorescence spectroscopy
Ryder, Alan G.; Calvet, Amandine; Li, Boyan (2012)The rapid and inexpensive analysis of the complex cell culture media used in industrial mammalian cell culture is required for quality and variance monitoring. Excitation-emission matrix (EEM) spectroscopy combined with ... -
Solid-state transformations of sulfathiazole polymorphs: The effects of milling and humidity
Hu, Yun; Erxleben, Andrea; Hodnett, Kieran; Li, Boyan; McArdle, Patrick; Rasmuson, Åke C.; Ryder, Alan G. (American Chemical Society, 2013-07-11)The effect of milling on the transitions of sulfathiazole polymorphs in the absence and presence of solvent and excipients was monitored by X-ray powder diffraction (XRPD), attenuated total reflectance infrared (ATR-IR), ... -
A stainless steel multi-well plate (SS-MWP) for high-throughput Raman analysis of dilute solutions
Ryder, Alan G.; Li, Boyan; Ryan, Paul W.; Sirimuthu, Narayana M. S. (Wiley, 2010)The use of Raman spectroscopy for the qualitative and quantitative analysis of dilute aqueous solutions is of interest to the biopharmaceutical manufacturing sector. However, the inherent weakness of the Raman effect, ... -
Using surface-enhanced Raman scattering (SERS) and fluorescence spectroscopy for screening yeast extracts, a complex component of cell culture media
Ryder, Alan G.; Li, Boyan; Sirimithu, Narayana M.S.; Ray, Bryan H. (2012)Yeastolate or yeast extract, which are hydrolysates produced by autolysis of yeast, are often employed as a raw material in the media used for industrial mammalian cell culture. The source and quality of yeastolate can ...