Browsing School of Chemistry by Author "Casamayou-Boucau, Yannick"
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Accurate anisotropy recovery from fluorophore mixtures using Multivariate Curve Resolution (MCR)
Casamayou-Boucau, Yannick; Ryder, Alan G. (Elsevier, 2017-11-22)Anisotropy resolved multidimensional emission spectroscopy (ARMES) provides valuable insights into multi-fluorophore systems like proteins that have complex overlapping emission bands. The method combines multidimensional ... -
Advanced spectroscopy and APBS modeling for determination of the role of His190 and Trp103 in mouse thymidylate synthase interaction with selected dUMP analogues
Prokopowicz, Małgorzata; Jarmuła, Adam; Casamayou-Boucau, Yannick; Gordon, Fiona; Ryder, Alan; Sobich, Justyna; Maj, Piotr; Cieśla, Joanna; Zieliński, Zbigniew; Fita, Piotr; Rode, Wojciech (MDPI, 2021-03-06)A homo-dimeric enzyme, thymidylate synthase (TS), has been a long-standing molecular target in chemotherapy. To further elucidate properties and interactions with ligands of wild-type mouse thymidylate synthase (mTS) and ... -
Chemometric approaches to low-content quantification (LCQ) in solid-state mixtures using Raman mapping spectroscopy.
Li, Boyan; Casamayou-Boucau, Yannick; Calvet, Amandine; Ryder, Alan G. (Royal Society of Chemistry, 2017-10-30)The low-content quantification (LCQ) of active pharmaceutical ingredients or impurities in solid mixtures is important in pharmaceutical manufacturing and analysis. We previously demonstrated the feasibility of using Raman ... -
Evaluating the interaction of human serum albumin (HSA) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) liposomes in different aqueous environments using anisotropy resolved multi-dimensional emission spectroscopy (ARMES)
Gordon, Fiona; Casamayou-Boucau, Yannick; Ryder, Alan G. (Elsevier, 2022-01-07)Studying the interaction between plasma proteins and liposomes is critical, particularly for their use as drug delivery systems. Here, the efficacy of anisotropy resolved multidimensional emission spectroscopy (ARMES) for ... -
Extended wavelength anisotropy resolved multidimensional emission spectroscopy (ARMES) measurements: better filters, validation standards, and Rayleigh scatter removal methods
Casamayou-Boucau, Yannick; Ryder, Alan G. (IOP Publishing, 2017-08-17)Anisotropy resolved multidimensional emission spectroscopy (ARMES) provides valuable insights into multi-fluorophore proteins (Groza et al 2015 Anal. Chim. Acta 886 133-42). Fluorescence anisotropy adds to the multidimensional ... -
Investigating native state fluorescence emission of Immunoglobulin G using polarized Excitation Emission Matrix (pEEM) spectroscopy and PARAFAC
Steiner-Browne, Marina; Elcoroaristizabal, Saioa; Casamayou-Boucau, Yannick; Ryder, Alan G. (Elsevier, 2018-12-20)Intrinsic fluorescence spectroscopy (IFS) measurements for protein structural analysis can be enhanced by the use of anisotropy resolved multidimensional emission spectroscopy (ARMES). ARMES attempts to overcome the ... -
Kernel principal component analysis residual diagnosis (KPCARD): an automated method to remove cosmic ray artefacts in Raman spectra.
Li, Boyan; Calvet, Amandine; Casamayou-Boucau, Yannick; Ryder, Alan G. (2016)A new, fully automated, rapid method, referred to as kernel principal component analysis residual diagnosis (KPCARD), is proposed for removing cosmic ray artifacts (CRAs) in Raman spectra, and in particular for large Raman ... -
Low-content quantification in powders using Raman spectroscopy: a facile chemometric approach to sub 0.1% limits of detection.
Li, Boyan; Calvet, Amandine; Ryder, Alan G.; Casamayou-Boucau, Yannick; Morris, Cheryl (American Chemical Society, 2015-02-23)A robust and accurate analytical methodology for low-content (<0.1%) quantification in the solid-state using Raman spectroscopy, sub-sampling, and chemometrics was demonstrated using a piracetam–proline model. The method ... -
Quantitative analysis of weakly bound insulin oligomers in solution using polarized multidimensional fluorescence spectroscopy
Casamayou-Boucau, Yannick; Ryder, Alan G. (Elsevier, 2020-09-08)Being able to measure the size and distribution of oligomers in solution is a critical issue in the manufacture and stability of insulin and other protein formulations. Measuring oligomers reliably can however be complicated, ...