Browsing School of Chemistry by Author "Elcoroaristizabal, Saioa"
Now showing items 1-7 of 7
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Characterization of Lysozyme PEGylation products using polarized Excitation Emission Matrix (pEEM) spectroscopy
de Faria E Silva, Ana Luiza; Elcoroaristizabal, Saioa; Ryder, Alan G. (Wiley, 2020-07-07)The growing use of therapeutic proteins requires accurate measurement techniques for measuring biophysical and structural changes during manufacturing. This is particularly true for PEGylation of proteins, because ... -
Development of a rapid Polarized Total Synchronous Fluorescence Spectroscopy (pTSFS) method for protein quantification in a model bioreactor broth
Boateng, Bernard O.; Elcoroaristizabal, Saioa; Ryder, Alan G. (Wiley, 2021-01-27)Protein quantification during bioprocess monitoring is essential for biopharmaceutical manufacturing and is complicated by the complex chemical composition of the bioreactor broth. Here we present the early-stage development ... -
An excitation emission fluorescence lifetime spectrometer using a frequency doubled supercontinuum laser source
Melnikau, Dzmitry; Elcoroaristizabal, Saioa; Ryder, Alan G. (IOP Publishing, 2018-09-03)The accurate fluorescence analysis of complex, multi-fluorophore containing proteins requires the use of multi-dimensional measurement techniques. For the measurement of intrinsic fluorescence from tyrosine (Tyr) and ... -
Investigating native state fluorescence emission of Immunoglobulin G using polarized Excitation Emission Matrix (pEEM) spectroscopy and PARAFAC
Steiner-Browne, Marina; Elcoroaristizabal, Saioa; Casamayou-Boucau, Yannick; Ryder, Alan G. (Elsevier, 2018-12-20)Intrinsic fluorescence spectroscopy (IFS) measurements for protein structural analysis can be enhanced by the use of anisotropy resolved multidimensional emission spectroscopy (ARMES). ARMES attempts to overcome the ... -
Modelling Förster resonance energy transfer (FRET) using anisotropy resolved multi-dimensional emission spectroscopy (ARMES)
Gordon, Fiona; Elcoroaristizabal, Saioa; Ryder, Alan G. (Elsevier, 2020-11-16)Background Förster Resonance Energy Transfer (FRET) is widely used to study the structure and dynamics of biomolecular systems and also causes the non-linear fluorescence response observed in multi-fluorophore proteins. ... -
Multi-attribute quality screening of immunoglobulin G using polarized Excitation Emission Matrix spectroscopy
de Faria E Silva, Ana Luiza; Elcoroaristizabal, Saioa; Ryder, Alan G. (Elsevier, 2019-12-10)Immunoglobulin G (IgG) is often used as a starting material for the production of functionalised antibodies, like Antibody Drug Conjugates (ADCs), PEGlyated-conjugates, or radioimmunoconjugates. The gross structural quality ... -
Using polarized Total Synchronous Fluorescence Spectroscopy (pTSFS) with PARAFAC analysis for characterizing intrinsic protein emission
Steiner-Browne, Marina; Elcoroaristizabal, Saioa; Ryder, Alan G. (Elsevier, 2019-10-15)Using polarized Excitation Emission Matrix (pEEM) spectroscopy to measure the intrinsic emission of proteins offers a potentially useful methodology for a wide variety of potential applications. However, the presence of ...