Browsing School of Chemistry by Author "Ryder, Alan G."
Now showing items 41-60 of 65
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Monitoring cell culture media degradation using surface enhanced Raman scattering (SERS) spectroscopy
Calvet, Amandine; Ryder, Alan G. (Elsevier (Science Direct), 2014-06-13)The quality of the cell culture media used in biopharmaceutical manufacturing is a crucial factor affecting bioprocess performance and the quality of the final product. Due to their complex composition these media are ... -
Monitoring local unfolding of bovine serum albumin during denaturation using steady-state and time-resolved fluorescence spectroscopy
Togashi, Denisio M.; Ryder, Alan G.; O'Shaughnessy, Domhnall (Springer, 2010-03)In a previous report (J. Fluoresc. 16, 153, 2006) we studied the chaotropiclly induced denaturation of Bovine Serum Albumin (BSA) using the fluorescence decay kinetics at different stages in the denaturation of BSA by ... -
Multi-attribute quality screening of immunoglobulin G using polarized Excitation Emission Matrix spectroscopy
de Faria E Silva, Ana Luiza; Elcoroaristizabal, Saioa; Ryder, Alan G. (Elsevier, 2019-12-10)Immunoglobulin G (IgG) is often used as a starting material for the production of functionalised antibodies, like Antibody Drug Conjugates (ADCs), PEGlyated-conjugates, or radioimmunoconjugates. The gross structural quality ... -
Performance Monitoring of a Mammalian Cell Based Bioprocess using Raman spectroscopy.
Ryder, Alan G.; Li, Boyan; Ray, Bryan H. (2013)Being able to predict the final product yield at all stages in long-running, industrial, mammalian cell culture processes is vital for both operational efficiency, process consistency, and the implementation of Quality by ... -
Prediction of Cell Culture Media Performance Using Fluorescence Spectroscopy
Ryan, Paul W.; Boyan, Li; Shanahan, Michael; Ryder, Alan G. (2010)Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in ... -
Prediction of Cell Culture Media performance using Fluorescence Spectroscopy
Ryan, Paul W.; Li, Boyan; Shanahan, Michael; Ryder, Alan G. (ACS Publications, 2010)Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in ... -
Prediction of cell culture media performance using fluorescence spectroscopy.
Ryan, Paul W.; Li, Boyan; Shanahan, Michael; Ryder, Alan G. (American Chemical Society, 2010)Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in ... -
Quantitative analysis of cocaine in solid mixtures using Raman spectroscopy and chemometric methods
Ryder, Alan G.; O'Connor, Gerard M.; Thomas J., Glynn (Wiley, 2000)Near-infrared (785 mn) excitation was used to obtain Raman spectra from a series of 33 solid mixtures containing cocaine, caffeine and glucose (9.8-80.6% by weight cocaine), which were then analysed using chemometric ... -
Quantitative analysis of cocaine in solid mixtures using Raman spectroscopy and chemometric methods
Ryder, Alan G.; O'Connor, Gerard M.; Glynn, Thomas J. (Wiley, 2000-04-27)Near-infrared (785 mn) excitation was used to obtain Raman spectra from a series of 33 solid mixtures containing cocaine, caffeine and glucose (9.8-80.6% by weight cocaine), which were then analysed using chemometric ... -
Quantitative analysis of weakly bound insulin oligomers in solution using polarized multidimensional fluorescence spectroscopy
Casamayou-Boucau, Yannick; Ryder, Alan G. (Elsevier, 2020-09-08)Being able to measure the size and distribution of oligomers in solution is a critical issue in the manufacture and stability of insulin and other protein formulations. Measuring oligomers reliably can however be complicated, ... -
Quantitative polymorph contaminant analysis in tablets using Raman and near infra-red spectroscopies
Ryder, Alan G.; Hennigan, MC (2013)The detection and quantification of alternate polymorphs of active pharmaceutical ingredients (APIs), particularly at low concentrations is a key issue for the manufacture and analysis of solid-state formulations. Each ... -
Rapid characterization and quality control of complex cell culture media solutions using Raman spectroscopy and chemometrics
Li, Boyan; Ryan, Paul W.; Ray, Bryan H.; Sirimuthu, Narayana M. S.; Ryder, Alan G. (2010)The use of Raman spectroscopy coupled with chemometrics for the rapid identification, characterization, and quality assessment of Complex cell culture media components used for industrial mammalian cell culture was ... -
A rapid fluorescence based method for the quantitative analysis of cell culture media photo-degradation
Ryder, Alan G. (Elsevier (Science Direct), 2013-11-21)Cell culture media are very complex chemical mixtures that are one of the most important aspects in biopharmaceutical manufacturing. The complex composition of many media leads to materials that are inherently unstable and ... -
Rapid quantification of tryptophan and tyrosine in chemically defined cell culture media using fluorescence spectroscopy
Ryder, Alan G.; Calvet, Amandine; Li, Boyan (2012)The rapid and inexpensive analysis of the complex cell culture media used in industrial mammalian cell culture is required for quality and variance monitoring. Excitation-emission matrix (EEM) spectroscopy combined with ... -
Size exclusion chromatography for screening yeastolate used in cell culture media
Kyne, Michelle; de Faria e Silva, Ana Luiza; Vickroy, Bruce; Ryder, Alan G. (Elsevier, 2023-09-18)Yeastolate is often used as a media supplement in industrial mammalian cell culture or as a major media component for microbial fermentations. Yeastolate variability can significantly affect process performance, but analysis ... -
Solid-state transformations of sulfathiazole polymorphs: The effects of milling and humidity
Hu, Yun; Erxleben, Andrea; Hodnett, Kieran; Li, Boyan; McArdle, Patrick; Rasmuson, Åke C.; Ryder, Alan G. (American Chemical Society, 2013-07-11)The effect of milling on the transitions of sulfathiazole polymorphs in the absence and presence of solvent and excipients was monitored by X-ray powder diffraction (XRPD), attenuated total reflectance infrared (ATR-IR), ... -
Solvothermal synthesis of discrete cages and extended networks comprising {Cr(III)3O(O2CR)3(oxime)3}2- (R = H, CH3, C(CH3)3, C14H9) building blocks
Houton, Edel; Comar, Priyanka; Pitak, Mateusz B.; Coles, Simon J.; Ryder, Alan G.; Piligkos, Stergios; Brechin, Euan K.; Jones, Leigh F. (Royal Society of Chemistry, 2016-07-28)The synthesis, structural and magnetic characterisation of a family of related Cr(III) cages are reported. Each member comprises {Cr(III)3O(O2CR1)3(R2-sao)3}2- (R1 = H, CH3, C(CH3)3, C14H9 ; R2 = Me, Ph, tBu, C10H8) triangles ... -
A stainless steel multi-well plate (SS-MWP) for high-throughput Raman analysis of dilute solutions
Ryder, Alan G.; Li, Boyan; Ryan, Paul W.; Sirimuthu, Narayana M. S. (Wiley, 2010)The use of Raman spectroscopy for the qualitative and quantitative analysis of dilute aqueous solutions is of interest to the biopharmaceutical manufacturing sector. However, the inherent weakness of the Raman effect, ... -
Study of water adsorption in PolyN-isopropylacrylamide) thin films using fluorescence emission of 3-hydroxyflavone probes
Morris, Cheryl; Szczupak, Boguslaw; Ryder, Alan G. (ACS Publications, 2010)The noncontact measurement of water uptake in microscale , thermoresponsive poly(N-isopropylacrylamide) thin films is challenging. We assessed the efficacy of three different 3-hydroxyflavone (3-HF)-based fluorophores to ... -
Super stable fluorescein isothiocyanate isomer I monolayer for total internal reflection fluorescence microscopy
Zarski, Przemyslaw; Ryder, Alan G. (American Chemical Society, 2018-08-27)Total internal reflection fluorescence microscopy (TIRFM) is an important method in surface science and for the analysis of surface-bound macromolecules. Here, we developed and explored the use of a novel fluorescein ...