Browsing by Subject "s-phase"
Now showing items 1-19 of 19
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A high through-put screen for small molecules modulating mcm2 phosphorylation identifies ryuvidine as an inducer of the dna damage response
(Public Library of Science (PLoS), 2014-06-05)DNA replication is an essential process for cell division and as such it is a process that is directly targeted by several anticancer drugs. CDC7 plays an essential role in the activation of replication origins and has ... -
Cdc7-dependent and -independent phosphorylation of claspin in the induction of the dna replication checkpoint
(Informa UK Limited, 2013-05-15)Claspin is a critical mediator protein in the DNA replication checkpoint, responsible for ATR-dependent activation of the effector kinase Chk1. Cdc7, an essential kinase required for the initiation of DNA replication, can ... -
Coordinated regulation of replication protein a activities by its subunits p14 and p32
(American Society for Biochemistry & Molecular Biology (ASBMB), 2004-06-17)The heterotrimeric replication protein A (RPA) has multiple essential activities in eukaryotic DNA metabolism and in signaling pathways. Despite extensive analyses, the functions of the smallest RPA subunit p14 are still ... -
Ddk dependent regulation of top2a at centromeres revealed by a chemical genetics approach
(Oxford University Press (OUP), 2016-07-12)In eukaryotic cells the CDC7/DBF4 kinase, also known as DBF4-dependent kinase (DDK), is required for the firing of DNA replication origins. CDC7 is also involved in replication stress responses and its depletion sensitises ... -
Distinctive activities of dna polymerases during human dna replication
(Wiley-Blackwell, 2006-07-01)The contributions of human DNA polymerases (pols) alpha, delta and epsilon during S-phase progression were studied in order to elaborate how these enzymes co-ordinate their functions during nuclear DNA replication. Pol ... -
Dna polymerase ε associates with the elongating form of rna polymerase ii and nascent transcripts
(Wiley-Blackwell, 2006-11-15)DNA polymerase epsilon co-operates with polymerases alpha and delta in the replicative DNA synthesis of eukaryotic cells. We describe here a specific physical interaction between DNA polymerase epsilon and RNA polymerase ... -
Dna polymerase η modulates replication fork progression and dna damage responses in platinum-treated human cells
(Springer Nature, 2013-11-20)Human cells lacking DNA polymerase eta (pol eta) are sensitive to platinum-based cancer chemotherapeutic agents. Using DNA combing to directly investigate the role of pol eta in bypass of platinum-induced DNA lesions in ... -
Double-strand breaks trigger mrx- and mec1-dependent, but tel1-independent, checkpoint activation
(Oxford University Press (OUP), 2006-08-01)Together with the Tel1 PI3 kinase, the Mre11/Rad50/Xrs2 (MRX) complex is involved in checkpoint activation in response to double-strand breaks (DSBs), a function also conserved in human cells by Mre11/Rad50/Nbs1 acting ... -
Hypoxic activation of atr and the suppression of the initiation of dna replication through cdc6 degradation
(Springer Nature, 2011-12-19)Many severely hypoxic cells fail to initiate DNA replication, but the mechanism underlying this observation is unknown. Specifically, although the ataxia-telangiectasia-rad3 related (ATR) kinase has been shown to be activated ... -
Interplay of dna damage and cell cycle signaling at the level of human replication protein a
(Elsevier BV, 2014-09-01) -
Microrna-29a regulates the benzo[a]pyrene dihydrodiol epoxide-induced dna damage response through cdc7 kinase in lung cancer cells
(Springer Nature, 2013-07-01)Cdc7 kinase is a key regulator of DNA replication and has an important role in the cellular DNA damage response by controlling checkpoint signaling and cell survival. Yet, how the activity of Cdc7 kinase is regulated is ... -
Oscillation of apc/c activity during cell cycle arrest promotes centrosome amplification
(The Company of Biologists, 2012-09-06)Centrosome duplication is licensed by the disengagement, or 'uncoupling', of centrioles during late mitosis. However, arrest of cells in G2 can trigger premature centriole disengagement. Here, we show that premature ... -
Parg is dispensable for recovery from transient replicative stress but required to prevent detrimental accumulation of poly(adp-ribose) upon prolonged replicative stress
(Oxford University Press (OUP), 2014-06-06)Poly(ADP-ribosyl)ation is involved in numerous biological processes including DNA repair, transcription and cell death. Cellular levels of poly(ADP-ribose) (PAR) are regulated by PAR polymerases (PARPs) and the degrading ... -
Promoter hijack reveals pericentrin functions in mitosis and the dna damage response
(Informa UK Limited, 2013-02-15)Centrosomes, the principal microtubule-organizing centers of animal somatic cells, consist of two centrioles embedded in the pericentriolar material (PCM). Pericentrin is a large PCM protein that is required for normal PCM ... -
Regulation of cdc45 in the cell cycle and after dna damage
(Portland Press Ltd., 2009-08-01)The Cdc (cell division cycle) 45 protein has a central role in the regulation of the initiation and elongation stages of eukaryotic chromosomal DNA replication. In addition, it is the main target for a Chk1 (checkpoint ... -
Regulation of eukaryotic dna replication at the initiation step
(Portland Press Ltd., 2003-02-01)The studies of cell growth and division have remained at the centre of biomedical research for more than 100 years. The combination of genetic, biochemical, molecular and cell biological techniques recently yielded a burst ... -
Repression of g1/s transcription is mediated via interaction of the gtb motifs of nrm1 and whi5 with swi6
(American Society for Microbiology, 2013-02-04)In Saccharomyces cerevisiae, G(1)/S transcription factors MBF and SBF regulate a large family of genes important for entry to the cell cycle and DNA replication and repair. Their regulation is crucial for cell viability, ... -
Role of the saccharomyces cerevisiae rad9 protein in sensing and responding to dna damage
(Portland Press Ltd., 2003-02-01)Eukaryotic cells have evolved surveillance mechanisms, known as DNA-damage checkpoints, that sense and respond to genome damage. DNA-damage checkpoint pathways ensure co-ordinated cellular responses to DNA damage, including ... -
The cell cycle regulator phosphorylated retinoblastoma protein is associated with tau pathology in several tauopathies
(Oxford University Press (OUP), 2011-07-01)Retinoblastoma protein (pRb) is a ubiquitous 928-amino acid cell cycle regulatory molecule with diverse biologic activities. One critical function of pRb is the control of the G(1)-to-S phase checkpoint of the cell cycle. ...