Browsing by Author "Joshi, Lokesh"
Now showing items 1-20 of 49
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A new microfluidic approach for the one-step capture, amplification and label-free quantification of bacteria from raw samples
Pereiro, Iago; Bendali, Amel; Tabnaoui, Sanae; Alexandre, Lucile; Srbova, Jana; Bilkova, Zuzana; Deegan, Shane; Joshi, Lokesh; Viovy, Jean-Louis; Malaquin, Laurent; Dupuy, Bruno; Descroix, Stéphanie (Royal Society of Chemistry (RSC), 2017-01-01)A microfluidic method to specifically capture and detect infectious bacteria based on immunorecognition and proliferative power is presented. It involves a microscale fluidized bed in which magnetic and drag forces are ... -
A preliminary study examining the binding capacity of akkermansia muciniphila and desulfovibrio spp., to colonic mucin in health and ulcerative colitis
Earley, Helen; Lennon, Grainne; Balfe, Aine; Kilcoyne, Michelle; Clyne, Marguerite; Joshi, Lokesh; Carrington, Stephen; Martin, Sean T.; Coffey, J. Calvin; Winter, Desmond C.; O’Connell, P. Ronan (Public Library of Science (PLoS), 2015-10-22)Background Akkermansia muciniphila and Desulfovibrio spp. are commensal microbes colonising the mucus gel layer of the colon. Both species have the capacity to utilise colonic mucin as a substrate. A. muciniphila degrades ... -
Campylobacter jejuni strain discrimination and temperature-dependent glycome expression profiling by lectin microarray
Kilcoyne, Michelle; Twomey, Marcus E.; Gerlach, Jared Q.; Kane, Marian; Moran, Anthony P.; Joshi, Lokesh (Elsevier, 2014-02-12)Gram-negative Campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans worldwide and the most frequently identified infectious trigger in patients developing Guillain-Barré syndrome (GBS). While ... -
Construction of a Natural Mucin Microarray and Interrogation for Biologically Relevant Glyco-Epitopes
Kilcoyne, Michelle; Gerlach, Jared Q.; Kane, Marian; Joshi, Lokesh (2012)Mucins are the principal components of mucus, and mucin glycosylation has important roles in defense, microbial adhesion, immunomodulation, inflammation, and cancer. Mucin expression and glycosylation are dynamic, responding ... -
Cross-feeding by bifidobacterium breve ucc2003 during co-cultivation with bifidobacterium bifidum prl2010 in a mucin-based medium
Egan, Muireann; O’Connell Motherway, Mary; Kilcoyne, Michelle; Kane, Marian; Joshi, Lokesh; Ventura, Marco; van Sinderen, Douwe (Springer Nature, 2014-11-25)Background: Bifidobacteria constitute a specific group of commensal bacteria that commonly inhabit the mammalian gastrointestinal tract. Bifidobacterium breve UCC2003 was previously shown to utilize a variety of ... -
Cross-platform comparison of glycan microarray formats
Wang, Linlin; Cummings, Richard D; Smith, David F; Huflejt, Margaret; Campbell, Christopher T; Gildersleeve, Jeffrey C; Gerlach, Jared Q; Kilcoyne, Michelle; Joshi, Lokesh; Serna, Sonia; Reichardt, Niels-Christian; Parera Pera, Núria; Pieters, Roland J; Eng, William; Mahal, Lara K (Oxford University Press (OUP), 2014-03-22)Carbohydrates participate in almost every aspect of biology from protein sorting to modulating cell differentiation and cell-cell interactions. To date, the majority of data gathered on glycan expression has been obtained ... -
Defatted bovine milk fat globule membrane inhibits association of enterohaemorrhagic escherichia coli o157:h7 with human ht-29 cells
Ross, Sarah A.; Lane, Jonathan A.; Kilcoyne, Michelle; Joshi, Lokesh; Hickey, Rita M. (Elsevier BV, 2016-08-01) -
Development and characterisation of recognition molecules for the specific identification of non-human glycans
Sharma, Shashank (2015-01-26)Glycosylation is an enzymatic process through which glycans attach to proteins, lipids and other organic molecules with huge structural diversity. This is a common post-translational modification found on proteins and cell ... -
Differential release of high mannose structural isoforms by fungal and bacterial endo-β-N-acetylglucosaminidases
Gerlach, Jared Q.; Kilcoyne, Michelle; Farrell, Mark P.; Kane, Marian; Joshi, Lokesh (Royal Society of Chemistry, 2012-02-28)Endo-beta-N-acetylglucosaminidases (ENGases) are widely used to remove N-linked oligosaccharides from glycoproteins for glycomic and proteomic studies and biopharmaceutical processes. Although several ENGases are widely ... -
Distinctive surface glycosylation patterns associated with mouse and human cd4+ regulatory t cells and their suppressive function
Cabral, Joana; Hanley, Shirley A.; Gerlach, Jared Q.; O’Leary, Neil; Cunningham, Stephen; Ritter, Thomas; Ceredig, Rhodri; Joshi, Lokesh; Griffin, Matthew D. (Frontiers Media SA, 2017-08-21)Regulatory T-cells (Treg) are essential for maintaining immune homeostasis and tolerance. Surface glycosylation is ubiquitous on mammalian cells and regulates diverse biological processes. While it is currently well accepted ... -
Donor bone marrow-derived dendritic cells prolong corneal allograft survival and promote an intragraft immunoregulatory milieu.
O'Flynn, Lisa; Treacy, Oliver; Ryan, Aideen E.; Morcos, Maurice; Cregg, Marese; Gerlach, Jared; Joshi, Lokesh; Nosov, Mikhail; Ritter, Thomas (Nature Publishing Group, 2013-08)Investigations into cell therapies for application in organ transplantation have grown. Here, we describe the ex vivo generation of donor bone marrow-derived dendritic cells (BMDCs) and glucocorticoid-treated BMDCs with ... -
Donor bone marrow–derived dendritic cells prolong corneal allograft survival and promote an intragraft immunoregulatory milieu
O'Flynn, Lisa; Treacy, Oliver; Ryan, Aideen E; Morcos, Maurice; Cregg, Marese; Gerlach, Jared; Joshi, Lokesh; Nosov, Mikhail; Ritter, Thomas (Elsevier BV, 2013-11-01)Investigations into cell therapies for application in organ transplantation have grown. Here, we describe the ex vivo generation of donor bone marrow-derived dendritic cells (BMDCs) and glucocorticoid-treated BMDCs with ... -
Examination of oestrus-dependent alterations of bovine cervico-vaginal mucus glycosylation for potential as optimum fertilisation indicators
Le Berre, Marie; Gerlach, Jared Q.; Loughrey, Catherine; Creavin, Aileen; Pluta, Katarzyna; Gallagher, Mary; Carrington, Stephen D.; Joshi, Lokesh; Kilcoyne, Michelle (Royal Society of Chemistry, 2021-03-09)Oestrus is the period in the sexual cycle of female mammals during which ovulation occurs, where they become most receptive to mating and are most fertile. Efficient detection of oestrus is a key component in successful ... -
Exploitation of spr to investigate the importance of glycan chains in the interaction between lactoferrin and bacteria
O’Riordan, Noelle; Kilcoyne, Michelle; Joshi, Lokesh; Hickey, Rita (MDPI AG, 2017-06-27)Bovine lactoferrin (LF) has been shown to prevent adhesion to and invasion of mammalian cell lines by pathogenic bacteria, with evidence for direct bacterial binding by the milk glycoprotein. However, the glycosylation ... -
Exploring the role of glycosylation in vascular biology, immunology,and stem cell biology
Gill, Satbir Kaur (2016-09-30)Carbohydrates are important modulators in various biological and physiological processes. They are present ubiquitously and coat all cells, forming the glycocalyx of the cell. Glycosylation, a type of post-translational ... -
Exposure of bifidobacterium longum subsp. infantis to milk oligosaccharides increases adhesion to epithelial cells and induces a substantial transcriptional response
Kavanaugh, Devon W.; O’Callaghan, John; Buttó, Ludovica F.; Slattery, Helen; Lane, Jonathan; Clyne, Marguerite; Kane, Marian; Joshi, Lokesh; Hickey, Rita M. (Public Library of Science (PLoS), 2013-06-21)In this study, we tested the hypothesis that milk oligosaccharides may contribute not only to selective growth of bifidobacteria, but also to their specific adhesive ability. Human milk oligosaccharides (3'sialyllactose ... -
Extracellular vesicles from paracoccidioides pathogenic species transport polysaccharide and expose ligands for dc-sign receptors
da Silva, Roberta Peres; Heiss, Christian; Black, Ian; Azadi, Parastoo; Gerlach, Jared Q.; Travassos, Luiz R.; Joshi, Lokesh; Kilcoyne, Michelle; Puccia, Rosana (Springer Nature, 2015-09-21)Extracellular vesicles (EVs) mediate non-conventional transport of molecules across the fungal cell wall. We aimed at describing the carbohydrate composition and surface carbohydrate epitopes of EVs isolated from the ... -
Fractionation of whey protein glycosylation and assessment of prebiotic and immunomodulation bioactivities for valorisation
Asadi Shehni, Akram (NUI Galway, 2021-03-15)Functional foods are defined as food components that, in addition to their nutritional value, improve human health and/or decrease the risk of diseases. Probiotics, ingested live beneficial bacteria, and prebiotics, the ... -
Gastrointestinal symptoms in Autism Spectrum Disorder: A systematic review
Leader, Geraldine; Abberton, Cathal; Cunningham, Stephen; Gilmartin, Katie; Grudzien, Margo; Higgins, Emily; Joshi, Lokesh; Whelan, Sally; Mannion, Arlene (MDPI, 2022-04-01)This systematic review aims to offer an updated understanding of the relationship between gastrointestinal symptoms (GIS) and autism spectrum disorder (ASD) in children and adolescents. The databases PsycINFO, Medline, ... -
Generation of recombinant anti-N-glycolylneuraminic acid (Neu5Gc) scFv and its applications for detection and quantitation of Neu5Gc
Sharma, Rajesh Kumar (2016-05-12)Sialic acids are a family of 9 carbon α-keto acid monosaccharides. Owing to their terminal position on glycans, sialic acids are involved in cell adhesion and recognition events, host pathogen interactions, immune regulation ...