Browsing by Author "Kyne, Ciara"
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Grasping the nature of the cell interior: fromphysiological chemistrytochemical biology
Kyne, Ciara; Crowley, Peter B. (Wiley-Blackwell, 2016-05-11)Current models of the cell interior emphasise its crowded, chemically complex and dynamically organised structure. Although the chemical composition of cells is known, the cooperative intermolecular interactions that govern ... -
Physicochemical properties of cells and their effects on intrinsically disordered proteins (idps)
Theillet, Francois-Xavier; Binolfi, Andres; Frembgen-Kesner, Tamara; Hingorani, Karan; Sarkar, Mohona; Kyne, Ciara; Li, Conggang; Crowley, Peter B.; Gierasch, Lila; Pielak, Gary J.; Elcock, Adrian H.; Gershenson, Anne; Selenko, Philipp (American Chemical Society (ACS), 2014-07-09) -
Protein charge determination and implications for interactions in cell extracts
Kyne, Ciara; Jordon, Kiara; Filoti, Dana I.; Laue, Thomas M.; Crowley, Peter B. (Wiley-Blackwell, 2016-12-01)Decades of dilute-solution studies have revealed the influence of charged residues on protein stability, solubility and stickiness. Similar characterizations are now required in physiological solutions to understand the ... -
Protein interactions in physiological environments
Kyne, Ciara (2016-06-03)The interactions of three proteins (ΔTat-GB1, cytochrome c and flavodoxin) were explored in Escherichia coli cells and extracts. The results indicate features of the physicochemical basis for protein assembly in the complex ... -
Specific ion effects on macromolecular interactions inescherichia coliextracts
Kyne, Ciara; Ruhle, Brian; Gautier, Virginie W.; Crowley, Peter B. (Wiley-Blackwell, 2014-12-30)Protein characterization in situ remains a major challenge for protein science. Here, the interactions of Tat-GB1 in Escherichia coli cell extracts were investigated by NMR spectroscopy and size exclusion chromatography ...