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Effects of electropermeabilization on the association of foreign dna with pig sperm
(Informa UK Limited, 1992-01-01)
This study was conducted to evaluate the effects of an electric pulse (electroporation/electropermeabilization) on the binding of foreign DNA molecules to porcine spermatozoa. We previously examined various parameters ...
Association of foreign dna with porcine spermatozoa
(Informa UK Limited, 1991-01-01)
The aim of this project was to establish if DNA molecules bind to procine spermatozoa after incubation in vitro. In an initial experiment, the DNA was mixed with porcine spermatozoa to test for the presence of DNases. ...
The fate of foreign dna associated with pig sperm following the in vitro fertilization of zona-free hamster ova and zona-intact pig ova
(Informa UK Limited, 1992-01-01)
The aim of this study was to determine the fate of foreign DNA molecules bound to porcine sperm that had been capacitated and acrosome reacted in vitro using calcium ionophore and then used in the in vitro fertilization ...
Improved efficiency for primer extension by using a long, highly-labeled primer generated from immobilized single-stranded dna templates
(Oxford University Press (OUP), 1997-04-01)
Primer extension is one of the most common methods used to measure the amount and size of RNAs. We demonstrate that the sensitivity and the specificity of this method are improved considerably by using a highly-labeled ...