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dc.contributor.authorSmith, Richard W. P.
dc.contributor.authorNasheuer, Heinz-Peter
dc.date.accessioned2018-08-24T08:26:26Z
dc.date.available2018-08-24T08:26:26Z
dc.date.issued2003-05-01
dc.identifier.citationSmith, Richard W. P. Nasheuer, Heinz-Peter (2003). Initiation of jc virus dna replication in vitro by human and mouse dna polymerase alpha-primase. European Journal of Biochemistry 270 (9), 2030-2037
dc.identifier.issn0014-2956,1432-1033
dc.identifier.urihttp://hdl.handle.net/10379/9893
dc.description.abstractHost species specificity of the polyomaviruses simian virus 40 (SV40) and mouse polyomavirus (PyV) has been shown to be determined by the host DNA polymerase alpha-primase complex involved in the initiation of both viral and host DNA replication. Here we demonstrate that DNA replication of the related human pathogenic polyomavirus JC virus (JCV) can be supported in vitro by DNA polymerase alpha-primase of either human or murine origin indicating that the mechanism of its strict species specificity differs from that of SV40 and PyV. Our results indicate that this may be due to differences in the interaction of JCV and SV40 large T antigens with the DNA replication initiation complex.
dc.publisherWiley-Blackwell
dc.relation.ispartofEuropean Journal of Biochemistry
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectDNA replication
dc.subjectinitiation
dc.subjectDNA polymerase alpha-primase
dc.subjectspecies specificity
dc.subjectpolyomavirus
dc.subjecthuman polyomavirus-jc
dc.subjectlarge t-antigen
dc.subjectprogressive multifocal leukoencephalopathy
dc.subjectspecies-specific replication
dc.subjectfunctional-characterization
dc.subjectregulatory region
dc.subjectcomplex-formation
dc.subjectsv40 origin
dc.subjectp48 control
dc.subjecthost-range
dc.titleInitiation of jc virus dna replication in vitro by human and mouse dna polymerase alpha-primase
dc.typeArticle
dc.identifier.doi10.1046/j.1432-1033.2003.03579.x
dc.local.publishedsourcehttp://onlinelibrary.wiley.com/doi/10.1046/j.1432-1033.2003.03579.x/pdf
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