Effect of helicobacter pylori lipopolysaccharide (lps) and lps derivatives on the production of tissue factor and plasminogen activator inhibitor type 2 by human blood mononuclear cells
Moran, A. P.
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Semeraro, N. Montemurro, P.; Piccoli, C.; Muoio, V.; Colucci, M.; Giuliani, G.; Fumarola, D.; Pece, S.; Moran, A. P. (1996). Effect of helicobacter pylori lipopolysaccharide (lps) and lps derivatives on the production of tissue factor and plasminogen activator inhibitor type 2 by human blood mononuclear cells. Journal of Infectious Diseases 174 (6), 1255-1260
Different Helicobacter pylori lipopolysaccharides (LPSs) and LPS-derivatives were studied for their ability to induce the production of procoagulant activity (PCA) and plasminogen activator inhibitor type 2 (PAI-2) by human blood mononuclear leukocytes, Smooth (S)- and rough (R)-form LPSs caused a similar increase in cell-associated PCA (tissue factor) and PAI-2 antigen release. Both effects were potentiated by fetal bovine serum via a CD14-mediated mechanism, The potency of H. pylori LPSs was similar to 1000-fold lower than that of Salmonella typhimurium LPSs. When H. pylori LPS derivatives (dephosphorylated R-LPS, S-lipid A, and R-lipid A) were used, PCA and PAI-2 production were markedly reduced, R-lipid A was similar to 4-fold less efficient than S-lipid A, These findings suggest that the induction of monocyte tissue factor and PAI-2 by H. pylori LPS is influenced by the lipid A structure and modulated by the core oligosaccharide and that phosphate groups present in both regions may play an important role.