Show simple item record

dc.contributor.authorO'Connor, D.
dc.contributor.authorHynes, P.
dc.contributor.authorCormican, M.
dc.contributor.authorCollins, E.
dc.contributor.authorCorbett-Feeney, G.
dc.contributor.authorCassidy, M.
dc.date.accessioned2018-08-24T08:25:53Z
dc.date.available2018-08-24T08:25:53Z
dc.date.issued2001-08-01
dc.identifier.citationO'Connor, D. Hynes, P.; Cormican, M.; Collins, E.; Corbett-Feeney, G.; Cassidy, M. (2001). Evaluation of methods for detection of toxins in specimens of feces submitted for diagnosis of clostridium difficile- associated diarrhea. Journal of Clinical Microbiology 39 (8), 2846-2849
dc.identifier.issn0095-1137
dc.identifier.urihttp://hdl.handle.net/10379/9635
dc.description.abstractClostridium difficile is the principal pathogen associated with hospital-acquired acute diarrheal disease. We have evaluated the performances of six approaches for diagnosis of C. difficile-associated diarrhea (CDAD). Consecutive stool specimens (n = 200) from 133 patients were examined by cytotoxin assay, by culture of C. difficile on cycloserine-cefoxitin-fructose agar, and by toxin detection using four rapid immunoassay systems (Oxoid Toxin A test, ImmunoCard Toxin A test, TechLab Tox A/B II test, and Premier Toxins A&B test). A diagnosis of CDAD was established for 35 (27%) patients (representing 29% of specimens). The adjusted sensitivity and specificity of the methods were, respectively, 98 and 99% for the cytotoxin assay, 54 and 99% for ImmunoCard, 50 and 98% for Oxoid, 79 and 98% for TechLab, 80 and 98% for Premier, and 57 and 100% for culture. The TechLab and Premier assays are acceptable tests for diagnosis of CDAD but are not equivalent to the cytotoxin assay.
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofJournal of Clinical Microbiology
dc.subjectmulticenter evaluation
dc.subjectcytotoxicity assay
dc.subjecta/b test
dc.subjectculture
dc.subjectcolitis
dc.subjectinfection
dc.subjectstools
dc.subjectstrain
dc.titleEvaluation of methods for detection of toxins in specimens of feces submitted for diagnosis of clostridium difficile- associated diarrhea
dc.typeArticle
dc.identifier.doi10.1128/jcm.39.8.2846-2849.2001
dc.local.publishedsourcehttp://jcm.asm.org/content/39/8/2846.full.pdf
nui.item.downloads0


Files in this item

This item appears in the following Collection(s)

Show simple item record