dc.contributor.author | Murray, E. | |
dc.date.accessioned | 2018-08-24T08:25:50Z | |
dc.date.available | 2018-08-24T08:25:50Z | |
dc.date.issued | 2002-03-01 | |
dc.identifier.citation | Murray, E. (2002). Expression of surfactant protein d in the human gastric mucosa and during helicobacter pylori infection. Infection and Immunity 70 (3), 1481-1487 | |
dc.identifier.issn | 0019-9567 | |
dc.identifier.uri | http://hdl.handle.net/10379/9613 | |
dc.description.abstract | Helicobacter pylori establishes persistent infection of gastric mucosa with diverse clinical outcomes. The innate immune molecule surfactant protein D (SP-D) binds selectively to microorganisms, inducing aggregation and phagocytosis. In this study, we demonstrated the expression of SP-D in gastric mucosa by reverse transcription-PCR and immuohistochemical analysis. SP-D is present at the luminal surface and within the gastric pits, with maximal expression at the surface. Levels of expression are significantly increased in H. pylori-associated gastritis compared to those in the normal mucosa. Immunofluorescence microscopy was used to demonstrate binding and agglutination of H. pylori by SP-D in a lectin-specific manner. These activities resulted in a 50% reduction in the motility of H. pylori, as judged on the basis of curvilinear velocity measured by using a Hobson BacTracker. Lipopolysaccharides extracted from three H. pylori strains were shown to bind SP-D in a concentration-dependent manner, and there was marked variation in the avidity of binding among the strains. SP-D may therefore play a significant role in the innate immune response to H. pylori infection. | |
dc.publisher | American Society for Microbiology | |
dc.relation.ispartof | Infection and Immunity | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | alveolar macrophages | |
dc.subject | aspergillus-fumigatus | |
dc.subject | escherichia-coli | |
dc.subject | binding-protein | |
dc.subject | motility | |
dc.subject | lipopolysaccharides | |
dc.subject | phagocytosis | |
dc.subject | colonization | |
dc.subject | morphology | |
dc.subject | allergens | |
dc.title | Expression of surfactant protein d in the human gastric mucosa and during helicobacter pylori infection | |
dc.type | Article | |
dc.identifier.doi | 10.1128/iai.70.3.1481-1487.2002 | |
dc.local.publishedsource | http://iai.asm.org/content/70/3/1481.full.pdf | |
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