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    Expression of surfactant protein d in the human gastric mucosa and during helicobacter pylori infection

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    Date
    2002-03-01
    Author
    Murray, E.
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    Cited 44 times in Scopus (view citations)
    
    Recommended Citation
    Murray, E. (2002). Expression of surfactant protein d in the human gastric mucosa and during helicobacter pylori infection. Infection and Immunity 70 (3), 1481-1487
    Published Version
    http://iai.asm.org/content/70/3/1481.full.pdf
    Abstract
    Helicobacter pylori establishes persistent infection of gastric mucosa with diverse clinical outcomes. The innate immune molecule surfactant protein D (SP-D) binds selectively to microorganisms, inducing aggregation and phagocytosis. In this study, we demonstrated the expression of SP-D in gastric mucosa by reverse transcription-PCR and immuohistochemical analysis. SP-D is present at the luminal surface and within the gastric pits, with maximal expression at the surface. Levels of expression are significantly increased in H. pylori-associated gastritis compared to those in the normal mucosa. Immunofluorescence microscopy was used to demonstrate binding and agglutination of H. pylori by SP-D in a lectin-specific manner. These activities resulted in a 50% reduction in the motility of H. pylori, as judged on the basis of curvilinear velocity measured by using a Hobson BacTracker. Lipopolysaccharides extracted from three H. pylori strains were shown to bind SP-D in a concentration-dependent manner, and there was marked variation in the avidity of binding among the strains. SP-D may therefore play a significant role in the innate immune response to H. pylori infection.
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    http://hdl.handle.net/10379/9613
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