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dc.contributor.authorMorris, D.
dc.contributor.authorO'Hare, C.
dc.contributor.authorGlennon, M.
dc.contributor.authorMaher, M.
dc.contributor.authorCorbett-Feeney, G.
dc.contributor.authorCormican, M.
dc.date.accessioned2018-08-24T08:25:46Z
dc.date.available2018-08-24T08:25:46Z
dc.date.issued2003-07-23
dc.identifier.citationMorris, D. O'Hare, C.; Glennon, M.; Maher, M.; Corbett-Feeney, G.; Cormican, M. (2003). Extended-spectrum  -lactamases in ireland, including a novel enzyme, tem-102. Antimicrobial Agents and Chemotherapy 47 (8), 2572-2578
dc.identifier.issn0066-4804,1098-6596
dc.identifier.urihttp://hdl.handle.net/10379/9589
dc.description.abstractOrganisms producing extended-spectrum beta-lactamases (ESBLs) have been reported in many countries, but there is no information on the prevalence of ESBL-producing members of the family Enterobacteriaceae in Ireland. A total of 925 isolates of ampicillin-resistant members of the Enterobacteriaceae were received from six hospitals in Ireland over a 3-year period from September 1996 to September 1999. Isolates were screened for ESBL production by the double-disk diffusion (DDD) method. DDD-positive isolates that were (i) confirmed as ESBL producers by National Committee for Clinical Laboratory Standards (NCCLS) confirmatory testing and (ii) susceptible to cefoxitin by disk diffusion were considered ESBL producers. By these criteria, 27 (3%) of the ampicillin-resistant members of the Enterobacteriaceae studied were categorized as ESBL producers. Molecular typing suggested that some intra- and interhospital spread of ESBL-producing isolates had occurred. DNA sequencing of amplified bla(TEM) and bla(SHV) genes resulted in the detection of a novel bla(TEM) ESBL gene, bla(TEM-102) in two isolates (Klebsiella pneumoniae and Enterobacter cloacae) received from the same hospital but isolated from different patients. The study suggests dissemination of ESBL-producing bacteria within the health care system in Ireland and emphasizes the need for measures to control such spread.
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofAntimicrobial Agents and Chemotherapy
dc.subjectgram-negative bacteria
dc.subjectklebsiella-pneumoniae
dc.subjectescherichia-coli
dc.subjecttransferable resistance
dc.subjectserratia-marcescens
dc.subjectcephalosporinase production
dc.subjectmolecular epidemiology
dc.subjectenterobacter-cloacae
dc.subjectidentification
dc.subjectsusceptibility
dc.titleExtended-spectrum  -lactamases in ireland, including a novel enzyme, tem-102
dc.typeArticle
dc.identifier.doi10.1128/aac.47.8.2572-2578.2003
dc.local.publishedsourcehttp://aac.asm.org/content/47/8/2572.full.pdf
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