dc.contributor.author | Lardner, Anne L. | |
dc.contributor.author | O'Donovan, Daniel J. | |
dc.date.accessioned | 2018-08-24T08:25:25Z | |
dc.date.available | 2018-08-24T08:25:25Z | |
dc.date.issued | 1998-06-01 | |
dc.identifier.citation | Lardner, Anne L. O'Donovan, Daniel J. (1998). Renal and hepatic nitrogen metabolism during nh4cl ingestion in protein-deprived rats. European Journal of Biochemistry 254 (2), 428-432 | |
dc.identifier.issn | 0014-2956,1432-1033 | |
dc.identifier.uri | http://hdl.handle.net/10379/9429 | |
dc.description.abstract | Three groups of rats were given either a standard protein diet, a protein-free diet, or a protein-free diet with the inclusion of 0.28 M NH4Cl in their drinking water, for 10 days. Body, liver and kidney masses were decreased similarly in the protein-free and protein-free NH4Cl groups. Ingestion of protein-free diet resulted in profound systemic acidosis in both groups, the simultaneous consumption of NH4Cl having no further effect. The activities of the urea-cycle enzymes carbamoyl-phosphate synthease, ornithine transcarbamoylase, arginosuccinate lyase and arginase were significantly reduced in the protein-foe group, and the simultaneous ingestion of NH4Cl had no further effect. These results indicate that ammonium ingestion does not prevent the decrease in urea cycle enzyme activities during a period of dietary-protein deprivation. Renal phosphate-dependent glutaminase activity was unchanged in the protein-free group, but was significantly higher with simultaneous NH4Cl consumption, suggesting that the renal adaptation to acid ingestion is not compromised by a lack of dietary protein. Urinary ammonia excretion also increased in rats consuming protein-free diet and NH4Cl. Urinary urea excretion was greater in rats receiving protein-free diet and NH4Cl than in rats receiving protein-free diet only, at all time-points examined. These data demonstrate that urea synthesis is driven primarily by the need to dispose of protein-derived ammonia rather than bicarbonate. | |
dc.publisher | Wiley-Blackwell | |
dc.relation.ispartof | European Journal of Biochemistry | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | protein deprivation | |
dc.subject | ammonium chloride | |
dc.subject | urea cycle | |
dc.subject | glutaminase | |
dc.subject | nitrogen metabolism | |
dc.subject | mitochondrial n-acetylglutamate | |
dc.subject | chronic respiratory-acidosis | |
dc.subject | base-balance invivo | |
dc.subject | urea synthesis | |
dc.subject | citrulline synthesis | |
dc.subject | dietary-protein | |
dc.subject | ammonia | |
dc.subject | ph | |
dc.subject | glutaminase | |
dc.subject | lactate | |
dc.title | Renal and hepatic nitrogen metabolism during nh4cl ingestion in protein-deprived rats | |
dc.type | Article | |
dc.identifier.doi | 10.1046/j.1432-1327.1998.2540428.x | |
dc.local.publishedsource | http://onlinelibrary.wiley.com/doi/10.1046/j.1432-1327.1998.2540428.x/pdf | |
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