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dc.contributor.authorHynes, Sean O.
dc.contributor.authorFerris, John A.
dc.contributor.authorSzponar, Bogumila
dc.contributor.authorWadstrom, Torkel
dc.contributor.authorFox, James G.
dc.contributor.authorO'Rourke, Jani
dc.contributor.authorLarsson, Lennart
dc.contributor.authorYaquian, Elisa
dc.contributor.authorLjungh, Asa
dc.contributor.authorClyne, Marguerite
dc.contributor.authorAndersen, Leif P.
dc.contributor.authorMoran, Anthony P.
dc.date.accessioned2018-08-24T08:25:12Z
dc.date.available2018-08-24T08:25:12Z
dc.date.issued2004-08-01
dc.identifier.citationHynes, Sean O. Ferris, John A.; Szponar, Bogumila; Wadstrom, Torkel; Fox, James G.; O'Rourke, Jani; Larsson, Lennart; Yaquian, Elisa; Ljungh, Asa; Clyne, Marguerite; Andersen, Leif P.; Moran, Anthony P. (2004). Comparative chemical and biological characterization of the lipopolysaccharides of gastric and enterohepatic helicobacters. Helicobacter 9 (4), 313-323
dc.identifier.issn1083-4389,1523-5378
dc.identifier.urihttp://hdl.handle.net/10379/9331
dc.description.abstractBackground. The lipopolysaccharide of Helicobacter pylori plays an important role in colonization and pathogenicity. The present study sought to compare structural and biological features of lipopolysaccharides from gastric and enterohepatic Helicobacter spp. not previously characterized. Materials and methods. Purified lipopolysaccharides from four gastric Helicobacter spp. (H. pylori, Helicobacter felis, Helicobacter bizzozeronii and Helicobacter mustelae) and four enterohepatic Helicobacter spp. (Helicobacter hepaticus, Helicobacter bilis, 'Helicobacter sp. flexispira' and Helicobacter pullorum) were structurally characterized using electrophoretic, serological and chemical methods. Results. Structural insights into all three moieties of the lipopolysaccharides, i.e. lipid A, core and O-polysaccharide chains, were gained. All species expressed lipopolysaccharides bearing an O-polysaccharide chain, but H. mustelae and H. hepaticus produced truncated semirough lipopolysaccharides. However, in contrast to lipopolysaccharides of H. pylori and H. mustelae, no blood group mimicry was detected in the other Helicobacter spp. examined. Intra-species, but not interspecies, fatty acid profiles of lipopolysaccharides were identical within the genus. Although shared lipopolysaccharide-core epitopes with H. pylori occurred, differing structural characteristics were noted in this lipopolysaccharide region of some Helicobacter spp. The lipopolysaccharides of the gastric helicobacters, H. bizzozeronii and H. mustelae, had relative Limulus amoebocyte lysate activities which clustered around that of H. pylori lipopolysaccharide, whereas H. bilis, 'Helicobacter sp. flexispira' and H. hepaticus formed a cluster with approximately 1000-10,000-fold lower activities. H. pullorum lipopolysaccharide had the highest relative Limulus amoebocyte lysate activity of all the helicobacter lipopolysaccharides (10-fold higher than that of H. pylori lipopolysaccharide), and all the lipopolysaccharides of enterohepatic Helicobacter spp. were capable of inducing nuclear factor-Kappa B(NF-kappaB) activation. Conclusions. The collective results demonstrate the structural heterogeneity and pathogenic potential of lipopolysaccharides of the Helicobacter genus as a group and these differences in lipopolysaccharides may be indicative of adaptation of the bacteria to different ecological niches.
dc.publisherWiley-Blackwell
dc.relation.ispartofHelicobacter
dc.subjecthelicobacter spp
dc.subjecthelicobacter pylori
dc.subjectgastric helicobacters
dc.subjectenterohepatic helicobacters
dc.subjectlipopolysaccharide
dc.subjectlipid a
dc.subjectdesorption mass-spectrometry
dc.subjectfactor-kappa-b
dc.subjectpylori lipopolysaccharide
dc.subjectform lipopolysaccharides
dc.subjectepithelial-cells
dc.subjectmustelae
dc.subjectstrain
dc.subjectinfection
dc.subjectmucosa
dc.subjectfelis
dc.titleComparative chemical and biological characterization of the lipopolysaccharides of gastric and enterohepatic helicobacters
dc.typeArticle
dc.identifier.doi10.1111/j.1083-4389.2004.00237.x
dc.local.publishedsourcehttp://lup.lub.lu.se/search/ws/files/2962071/624308.pdf
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