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dc.contributor.authorHeneghan, M. A.
dc.contributor.authorMcCarthy, C. F.
dc.contributor.authorJanulaityte, D.
dc.contributor.authorMoran, A. P.
dc.identifier.citationHeneghan, M. A. McCarthy, C. F.; Janulaityte, D.; Moran, A. P. (2001). Relationship of anti-lewis x and anti-lewis y antibodies in serum samples from gastric cancer and chronic gastritis patients to helicobacter pylori-mediated autoimmunity. Infection and Immunity 69 (8), 4774-4781
dc.description.abstractLewis (Le) antigens have been implicated in the pathogenesis of atrophic gastritis and gastric cancer in the setting of Helicobacter pylori infection, and H. pylori-induced anti-Le antibodies have been described that cross-react with the gastric mucosa of both mice and humans. The aim of this study was to examine the presence of anti-Le antibodies in patients with H. pylori infection and gastric cancer and to examine the relationships between anti-Le antibody production, bacterial Le expression, gastric histopathology, and host Le erythrocyte phenotype. Anti-Le antibody production and H. pylori Le expression were determined by enzyme-linked immunosorbent assay, erythrocyte Le phenotype was examined by agglutination assays, and histology was scored blindly. Significant levels of anti-Le(x) antibody (P < 0.0001, T = 76.4, DF = 5) and anti-Ley antibody (P < 0.0001, T = 73.05, DF = 5) were found in the sera of patients with gastric cancer and other H. pylori-associated pathology compared with H. pylori-negative controls. Following incubation of patient sera with synthetic Le glycoconjugates, anti-Le(x) and -Le(y) autoantibody binding was abolished. The degree of the anti-Le(x) and -Le(y) antibody response was unrelated to the host Le phenotype but was significantly associated with the bacterial expression of Le(x) (r = 0.863, r(2) = 0.745, P < 0.0001) and Ley (r = 0.796, r(2) = 0.634, P < 0.0001), respectively. Collectively, these data suggest that anti-Le antibodies are present in most patients with H. pylori infection, including those with gastric cancer, that variability exists in the strength of the anti-Le response, and that this response is independent of the host Le phenotype but related to the bacterial Le phenotype.
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofInfection and Immunity
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.subjectblood-group antigens
dc.subjectcore oligosaccharide regions
dc.subjecthost lewis
dc.subjectinflammatory response
dc.subjectmolecular mimicry
dc.titleRelationship of anti-lewis x and anti-lewis y antibodies in serum samples from gastric cancer and chronic gastritis patients to helicobacter pylori-mediated autoimmunity

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