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dc.contributor.authorHALVEY, Patrick J.
dc.contributor.authorWATSON, Walter H.
dc.contributor.authorHANSEN, Jason M.
dc.contributor.authorGO, Young-Mi
dc.contributor.authorSAMALI, Afshin
dc.contributor.authorJONES, Dean P.
dc.date.accessioned2018-08-24T08:25:03Z
dc.date.available2018-08-24T08:25:03Z
dc.date.issued2005-03-01
dc.identifier.citationHALVEY, Patrick J. WATSON, Walter H.; HANSEN, Jason M.; GO, Young-Mi; SAMALI, Afshin; JONES, Dean P. (2005). Compartmental oxidation of thiol–disulphide redox couples during epidermal growth factor signalling. Biochemical Journal 386 , 215-219
dc.identifier.issn0264-6021,1470-8728
dc.identifier.urihttp://hdl.handle.net/10379/9266
dc.description.abstractExogenously added ROS (reactive oxygen species) cause generalized oxidation of cellular components, whereas endogenously generated ROS induced by physiological stimuli activate discrete signal transduction pathways. Compartmentation is an important aspect of such pathways, but little is known about its role in redox signalling. We measured the redox states of cytosolic and nuclear Trx1 (thioredoxin-1) and mitochondrial Trx2 (thioredoxin-2) using redox Western blot methodologies during endogenous ROS production induced by EGF (epidermal growth factor) signalling. The glutathione redox state was measured by HPLC. Results showed that only cytosolic Trx1 undergoes significant oxidation. Thus EGF signalling involves subcellular compartmental oxidation of Trx1 in the absence of a generalized cellular oxidation.
dc.publisherPortland Press Ltd.
dc.relation.ispartofBiochemical Journal
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectepidermal growth factor (egf)
dc.subjectglutathione
dc.subjectredox signalling
dc.subjectthioredoxin-1
dc.subjectthioredoxin-2
dc.subjecthuman mitochondrial thioredoxin
dc.subjecthuman cell-lines
dc.subjectimmunohistochemical localization
dc.subjecthydrogen-peroxide
dc.subjectDNA-binding
dc.subjectstress
dc.subjectglutathione
dc.subjectactivation
dc.subjectendocytosis
dc.subjectexpression
dc.titleCompartmental oxidation of thiol–disulphide redox couples during epidermal growth factor signalling
dc.typeArticle
dc.identifier.doi10.1042/bj20041829
dc.local.publishedsourcehttp://www.biochemj.org/content/ppbiochemj/386/2/215.full.pdf
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