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dc.contributor.authorGuerry, P.
dc.date.accessioned2018-08-24T08:25:01Z
dc.date.available2018-08-24T08:25:01Z
dc.date.issued2002-02-01
dc.identifier.citationGuerry, P. (2002). Phase variation of campylobacter jejuni 81-176 lipooligosaccharide affects ganglioside mimicry and invasiveness in vitro. Infection and Immunity 70 (2), 787-793
dc.identifier.issn0019-9567
dc.identifier.urihttp://hdl.handle.net/10379/9254
dc.description.abstractThe outer cores of the lipooligosaccharides (LOS) of many strains of Campylobacter jejuni mimic human gangliosides in structure. A population of cells of C.jejuni strain 81-176 produced a mixture of LOS cores which consisted primarily of structures mimicking GM(2) and GM(3) gangliosides, with minor amounts of structures mimicking GD(11) and GD(2). Genetic analyses of genes involved in the biosynthesis of the outer core of C. Jejuni 81-176 revealed the presence of a homopolymeric tract of G residues within a gene encoding CgtA, an N-acetylgalactosaminyltransferase. Variation in the number of G residues within cgtA affected the length of the open reading frame, and these changes in cgtA corresponded to a change in LOS structure from GM(2) to GM(3) ganglioside mimicry. Site-specific mutation of cgtA in 81-176 resulted in a major LOS core structure that lacked GaINAc and resembled GM(3) ganglioside. Compared to wild-type 81-176, the cgtA mutant showed a significant increase in invasion of INT407 cells. In comparison, a site-specific mutation of the neuC1 gene resulted in the loss of sialic acid in the LOS core and reduced resistance to normal human serum but had no affect on invasion of INT407 cells.
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofInfection and Immunity
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectguillain-barre-syndrome
dc.subjectneisseria-gonorrhoeae
dc.subjectmolecular mimicry
dc.subjectlipo-oligosaccharide
dc.subjectserum resistance
dc.subjectlipopolysaccharides
dc.subjectcells
dc.subjectacid
dc.subjectcampylobacter-jejuni-81-176
dc.subjectidentification
dc.titlePhase variation of campylobacter jejuni 81-176 lipooligosaccharide affects ganglioside mimicry and invasiveness in vitro
dc.typeArticle
dc.identifier.doi10.1128/iai.70.2.787-793.2002
dc.local.publishedsourcehttp://iai.asm.org/content/70/2/787.full.pdf
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland