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dc.contributor.authorGilbert, Christopher S
dc.contributor.authorGreen, Catherine M
dc.contributor.authorLowndes, Noel F
dc.date.accessioned2018-08-24T08:24:56Z
dc.date.available2018-08-24T08:24:56Z
dc.date.issued2001-07-01
dc.identifier.citationGilbert, Christopher S; Green, Catherine M; Lowndes, Noel F (2001). Budding yeast rad9 is an atp-dependent rad53 activating machine. Molecular Cell 8 (1), 129-136
dc.identifier.issn1097-2765
dc.identifier.urihttp://hdl.handle.net/10379/9215
dc.description.abstractWe find budding yeast Rad9 in two distinct, large, and soluble complexes in cell extracts. The larger (greater than or equal to 850 kDa) complex, found in nondamaged cells, contains hypophosphorylated Rad9, whereas the smaller (560 kDa) complex, which forms after DNA damage, contains hyperphosphorylated Rad9 and Rad53. This smaller Rad9 complex is capable of catalyzing phosphorylation and release of active Rad53 kinase, a process requiring the kinase activity of Rad53. However, Mec1 and Tel1 are no longer required once the 560 kDa complex has been formed. We propose a model whereby Mec1/Tel1-dependent hyperphosphorylation of Rad9 results in formation of the smaller Rad9 complex and recruitment of Rad53. This complex then catalyzes activation of Rad53 by acting as a scaffold that brings Rad53 molecules into close proximity, facilitating Rad53 in trans autophosphorylation and subsequent release of activated Rad53.
dc.publisherElsevier BV
dc.relation.ispartofMolecular Cell
dc.subjectDNA-damage checkpoint
dc.subjectsaccharomyces-cerevisiae
dc.subjectreplication checkpoint
dc.subjectstrand breaks
dc.subjectbrca1
dc.subjectphosphorylation
dc.subjectprotein
dc.subjectcomplex
dc.subjectrepair
dc.subjectrecognition
dc.titleBudding yeast rad9 is an atp-dependent rad53 activating machine
dc.typeArticle
dc.identifier.doi10.1016/s1097-2765(01)00267-2
dc.local.publishedsourcehttps://doi.org/10.1016/s1097-2765(01)00267-2
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