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dc.contributor.authorCurtin, J.
dc.contributor.authorCormican, M.
dc.contributor.authorFleming, G.
dc.contributor.authorKeelehan, J.
dc.contributor.authorColleran, E.
dc.date.accessioned2018-08-24T08:24:31Z
dc.date.available2018-08-24T08:24:31Z
dc.date.issued2003-09-23
dc.identifier.citationCurtin, J. Cormican, M.; Fleming, G.; Keelehan, J.; Colleran, E. (2003). Linezolid compared with eperezolid, vancomycin, and gentamicin in an in vitro model of antimicrobial lock therapy for staphylococcus epidermidis central venous catheter-related biofilm infections. Antimicrobial Agents and Chemotherapy 47 (10), 3145-3148
dc.identifier.issn0066-4804,1098-6596
dc.identifier.urihttp://hdl.handle.net/10379/9032
dc.description.abstractCentral venous catheter (CVC)-related infection (CVC-RI) is a common complication of CVC use. The most common etiological agents of CVC-RI are gram-positive organisms, in particular, staphylococci. An in vitro model for the formation of biofilms by Staphylococcus epidermidis ATCC 35984 on polyurethane coupons in a modified Robbins device was established. Biofilm formation was confirmed by electron microscopy and was quantified by determination of viable counts. Mueller-Hinton broth was replaced with sterile physiological saline (control) or a solution of vancomycin (10 mg/ml), gentamicin (10 mg/ml), linezolid (2 mg/ml), or eperezolid (4 mg/ml). Viable counts were performed with the coupons after exposure to antimicrobials for periods of 24, 72, 168, and 240 h. The mean viable count per coupon following establishment of the biofilm was 4.6 x 10(8) CFU/coupon, and that after 14 days of exposure to physiological saline was 2.5 x 10(7) CFU/coupon. On exposure to vancomycin (10 mg/ml), the mean counts were 2.5 x 10(7) CFU/coupon at 24 h, 4.3 x 10(6) CFU/coupon at 72 h, 1.4 x 10(5) CFU/coupon at 168 h, and undetectable at 240 h. With gentamicin (10 mg/ml) the mean counts were 2.7 x 10(7) CFU/coupon at 24 h, 3.7 x 10(6) CFU/coupon at 72 h, 8.4 x 10(6) CFU/coupon at 168 h, and 6.5 x 10(6) CFU/coupon at 240 h. With linezolid at 2 mg/ml the mean counts were 7.1 x 10(5) CFU/coupon at 24 h and not detectable at 72, 168, and 240 h. With eperezolid (4 mg/ml) no viable cells were recovered after 168 h. These data suggest that linezolid (2 mg/ml) and eperezolid (4 mg/ml) achieve eradication of S. epidermidis biofilms more rapidly than vancomycin (10 mg/ml) and gentamicin (10 mg/ml).
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofAntimicrobial Agents and Chemotherapy
dc.subjectpseudomonas-aeruginosa biofilms
dc.subjectparenteral-nutrition
dc.subjectgrowth-rate
dc.subjecttobramycin
dc.subjectresistance
dc.subjectciprofloxacin
dc.subjectdiagnosis
dc.subjectculture
dc.subjecthome
dc.titleLinezolid compared with eperezolid, vancomycin, and gentamicin in an in vitro model of antimicrobial lock therapy for staphylococcus epidermidis central venous catheter-related biofilm infections
dc.typeArticle
dc.identifier.doi10.1128/aac.47.10.3145-3148.2003
dc.local.publishedsourcehttp://aac.asm.org/content/47/10/3145.full.pdf
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