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dc.contributor.authorCONNOLLY, Edward
dc.contributor.authorDONLON, John
dc.date.accessioned2018-08-24T08:24:27Z
dc.date.available2018-08-24T08:24:27Z
dc.date.issued2003-08-01
dc.identifier.citationCONNOLLY, Edward; DONLON, John (2003). Effects of dietary glycerol on the expression of pterin carbinolamine dehydratase in the rat. Biochemical Journal 373 , 993-997
dc.identifier.issn0264-6021,1470-8728
dc.identifier.urihttp://hdl.handle.net/10379/8998
dc.description.abstractEarlier studies have shown that the abundance of hepatic phenylalanine hydroxylase (PAH) diminishes to 60% of control values in rats fed with a diet composed of 40% (w/w) glycerol [Guerin, Walsh, Donlon and Kaufman (1998) Int. J. Biochem. Cell Biol. 30, 1047-1054]. In this experimental model, there are corresponding decreases in the hepatic concentrations of both the hydroxylase cofactor, tetrahydrobiopterin, and the nucleotide guanosine triphosphate. We now show that the cytoplasmic activities of hepatic pterin-4a-carbinolamine dehydratase (PCD) are also lower in these animals, by approx. 50% compared with control values. Immunoblotting confirmed a diminution of protein abundance in vivo. PCD also functions as a dimerization cofactor (DCoH) for the hepatocyte nuclear factor 1alpha (HNF1alpha) and the relative abundance of PCD/DCoH in the nucleus is also decreased. There is a small reduction in the mRNA levels for PAH and for PCD/DCoH in the glycerol-fed animals. In the kidney, there is also a diminution in the abundance of both PAH and PCD proteins. Hepatic GTP cyclohydrolase I activity was not altered and the abundance of hepatic HNF1alpha remained unchanged. HNF1alpha is required for the expression of PAH in the liver and our results support a role for PCD/DCoH, through its interaction with HNF1alpha, in regulating the expression of PAH.
dc.publisherPortland Press Ltd.
dc.relation.ispartofBiochemical Journal
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectcarbinolamine dehydratase
dc.subjectglycerol
dc.subjecthepatocyte nuclear factor 1 alpha
dc.subjectphenylalanine hydroxylation
dc.subjectregulation
dc.subjecttetrahydrobiopterin
dc.subjectphenylalanine-hydroxylase gene
dc.subjecthepatocyte nuclear factor-1
dc.subject4a-carbinolamine dehydratase
dc.subjectstimulating protein
dc.subjectcofactor
dc.subjectliver
dc.subjectpurification
dc.subjectsystem
dc.subjectdcoh
dc.subjecttetrahydrobiopterin
dc.titleEffects of dietary glycerol on the expression of pterin carbinolamine dehydratase in the rat
dc.typeArticle
dc.identifier.doi10.1042/bj20021345
dc.local.publishedsourcehttp://www.biochemj.org/content/ppbiochemj/373/3/993.full.pdf
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland