dc.contributor.author | Concannon, C.G. | |
dc.contributor.author | Orrenius, S. | |
dc.contributor.author | Samali, A. | |
dc.date.accessioned | 2018-08-24T08:24:25Z | |
dc.date.available | 2018-08-24T08:24:25Z | |
dc.date.issued | 2001-04-01 | |
dc.identifier.citation | Concannon, C.G. Orrenius, S.; Samali, A. (2001). Hsp27 inhibits cytochrome <i>c</i>-mediated caspase activation by sequestering both pro-caspase-3 and cytochrome <i>c</i>. Gene Expression 9 (4), 195-201 | |
dc.identifier.issn | 1052-2166,1555-3884 | |
dc.identifier.uri | http://hdl.handle.net/10379/8987 | |
dc.description.abstract | Mitochondrial cytochrome c release in response to pro-apoptotic signals leads to the formation of a cytochrome c/Apaf-1/procaspase-9 complex (the apoptosome) and resultant activation of caspase-9 and caspase-3. Here we demonstrate that the molecular chaperone, Hsp27, inhibits this cytochrome c-mediated activation of caspase-3. Immunodepeletion of Hsp27 from cytochrome c-activated cytosols resulted in decreased caspase activity. Furthermore, immunoprecipitation of Hsp27 resulted in the coprecipitation of both cytochrome c and procaspase-3. In reciprocal experiments, immunoprecipitation of both procaspase-3 and cytochrome c resulted in coprecipitation of Hsp27, indicating two independent interactions. These results point to Hsp27 mediating its inhibition of procaspase-3 activation through its ability to sequester both cytochrome c and procaspase-3, and thus prevent the correct formation/function of the apoptosome complex. | |
dc.publisher | Cognizant, LLC | |
dc.relation.ispartof | Gene Expression | |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | apoptosis | |
dc.subject | caspase | |
dc.subject | cytochrome c | |
dc.subject | hsp27 | |
dc.subject | stress | |
dc.subject | heat-shock proteins | |
dc.subject | small stress proteins | |
dc.subject | cell-death | |
dc.subject | dependent activation | |
dc.subject | oxidative stress | |
dc.subject | apoptosis | |
dc.subject | mitochondria | |
dc.subject | release | |
dc.subject | bcl-2 | |
dc.subject | procaspase-9 | |
dc.title | Hsp27 inhibits cytochrome <i>c</i>-mediated caspase activation by sequestering both pro-caspase-3 and cytochrome <i>c</i> | |
dc.type | Article | |
dc.identifier.doi | 10.3727/000000001783992605 | |
dc.local.publishedsource | http://europepmc.org/articles/pmc5964942?pdf=render | |
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