Evaluation of a pcr/dna probe colorimetric membrane assay for identification of campylobacter spp. in human stool specimens

Collins, E.; Glennon, M.; Hanley, S.; Murray, A.-M.; Cormican, M.; Smith, T.; Maher, M.

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2001-11-01

Author

Collins, E.

Glennon, M.

Hanley, S.

Murray, A.-M.

Cormican, M.

Smith, T.

Maher, M.

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Collins, E. Glennon, M.; Hanley, S.; Murray, A.-M.; Cormican, M.; Smith, T.; Maher, M. (2001). Evaluation of a pcr/dna probe colorimetric membrane assay for identification of campylobacter spp. in human stool specimens. Journal of Clinical Microbiology 39 (11), 4163-4165

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http://jcm.asm.org/content/39/11/4163.full.pdf

Abstract

DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCR/DNA probe positive for C jejuni. The assay is rapid and simple and can be applied to stool specimens for the detection of Campylobacter.

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http://hdl.handle.net/10379/8979

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