Evaluation of a pcr/dna probe colorimetric membrane assay for identification of campylobacter spp. in human stool specimens
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Collins, E. Glennon, M.; Hanley, S.; Murray, A.-M.; Cormican, M.; Smith, T.; Maher, M. (2001). Evaluation of a pcr/dna probe colorimetric membrane assay for identification of campylobacter spp. in human stool specimens. Journal of Clinical Microbiology 39 (11), 4163-4165
DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCR/DNA probe positive for C jejuni. The assay is rapid and simple and can be applied to stool specimens for the detection of Campylobacter.