Chemical structures of the core regions of campylobacter jejuni serotypes o:1, o:4, o:23, and o:36 lipopolysaccharides
ASPINALL, Gerald O.
McDONALD, Armando G.
RAJU, T. Shantha
MORAN, Anthony P.
PENNER, John L.
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ASPINALL, Gerald O. McDONALD, Armando G.; RAJU, T. Shantha; PANG, Henrianna; MORAN, Anthony P.; PENNER, John L. (1993). Chemical structures of the core regions of campylobacter jejuni serotypes o:1, o:4, o:23, and o:36 lipopolysaccharides. European Journal of Biochemistry 213 (3), 1017-1027
Complete structures, including the location of N-acetylneuraminic acid (Neu5Ac) residues, were assigned for the core regions of Campylobacter jejuni serotypes 0:1, 0:4, and 0:23 and 0:36 lipopolysaccharides (LPS). In continuation of earlier studies, structure determinations of liberated oligosaccharides and, where necessary, of intact LPS, were by H-1-NMR spectroscopy, Smith degradation, chromium trioxide and enzymic degradations, in conjunction With methylation studies supported by fast-atom-bombardment mass spectrometry and linkage analyses by gas chromatography/mass spectrometry. It was concluded on the basis of the following structures, in which each was linked 1-->5 to a terminal 3-deoxy-D-manno-octulosonic acid residue, that the core regions with qualititatively similar sugar compositions showed serotypic differences in one or more of their sequences, linkage types, and anometic configurations: [GRAPHICS] The outer regions of each structure carry Neu5Ac residues linked 2-->3 to available beta-D-Galp residues and show striking similarities with various glycosphingolipids of the ganglioside family. However, Neu5Ac epitopes are not apparently involved in determining serospecificity.