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dc.contributor.authorANNUK, HEIDI
dc.contributor.authorHIRMO, SIIRI
dc.contributor.authorMIKELSAAR, MARIKA
dc.contributor.authorWADSTRÖM, TORKEL
dc.contributor.authorHYNES, SEAN O.
dc.date.accessioned2018-08-24T08:24:01Z
dc.date.available2018-08-24T08:24:01Z
dc.date.issued2001-12-01
dc.identifier.citationANNUK, HEIDI; HIRMO, SIIRI; MIKELSAAR, MARIKA; WADSTRÖM, TORKEL; HYNES, SEAN O. (2001). Characterisation and differentiation of lactobacilli by lectin typing. Journal of Medical Microbiology 50 (12), 1069-1074
dc.identifier.issn0022-2615,1473-5644
dc.identifier.urihttp://hdl.handle.net/10379/8833
dc.description.abstractLactobacillus isolates from healthy Estonian and Swedish children were characterised by a lectin typing technique; 56 isolates from six species (L. acidophilus, L. paracasei, L. plantarum, L. fermentum, L. brevis and L. buchneri) were tested. The typing system was based on an agglutination assay with a panel of six commercially available lectins, which,were chosen on the basis of their carbohydrate specificities. The isolates were also subjected to proteolytic degradation before lectin typing to decrease auto-agglutination of whole cells in the assay. The 56 isolates were divided into 15 different lectin types by their lectin agglutination patterns. Proteolytic treatment reduced auto-agglutination for the majority of species, apart from L. acidophilus, which remained predominantly auto-agglutinating (eight of nine strains). The system produced stable and reproducible results under standardised culture conditions. Lactobacilli are important bacteria for use as probiotics and this system may supplement current molecular typing techniques and may help in identification of strains that could be useful in this role.
dc.publisherMicrobiology Society
dc.relation.ispartofJournal of Medical Microbiology
dc.subjectlactic-acid bacteria
dc.subjectbinding
dc.subjectidentification
dc.subjectassay
dc.titleCharacterisation and differentiation of lactobacilli by lectin typing
dc.typeArticle
dc.identifier.doi10.1099/0022-1317-50-12-1069
dc.local.publishedsourcehttp://jmm.microbiologyresearch.org/deliver/fulltext/jmm/50/12/mjm5012.1069.pdf?itemId=/content/journal/jmm/10.1099/0022-1317-50-12-1069&mimeType=pdf&isFastTrackArticle=
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