dc.contributor.author | Dorai-Raj, Siobhan | en |
dc.contributor.author | O' Grady, Justin | en |
dc.contributor.author | Colleran, Emer | en |
dc.date.accessioned | 2010-03-12T14:59:19Z | en |
dc.date.available | 2010-03-12T14:59:19Z | en |
dc.date.issued | 2009 | en |
dc.identifier.citation | Dorai-Raj, S., Grady, J. O., & Colleran, E. (2009). Specificity and sensitivity evaluation of novel and existing Bacteroidales and Bifidobacteria-specific PCR assays on feces and sewage samples and their application for microbial source tracking in Ireland. Water Research, 43(19), 4980-4988. | en |
dc.identifier.uri | http://hdl.handle.net/10379/782 | en |
dc.description.abstract | Three novel ruminant-specific PCR assays, an existing ruminant-specific PCR assay and five existing human-specific PCR assays, which target 16S rDNA from Bacteroidales or Bifidobacteria, were evaluated. The assays were tested on DNA extracted from ruminant (n = 74), human (n = 59) and non-ruminant animal (n = 44) sewage/fecal samples collected in Ireland. The three novel PCR assays compared favourably to the existing ruminant-specific assay, exhibiting sensitivities of 91 - 100% and specificities of 95 - 100% as compared to a sensitivity of 95% and specificity of 94%, for the existing ruminant-specific assay. Of the five human-specific PCR assays, the assay targeting the Bifidobacterium catenulatum group was the most promising, exhibiting a sensitivity of 100% (with human sewage samples) and a specificity of 87%. When tested on rural water samples that were naturally contaminated by ruminant feces, the three novel PCR assays tested positive with a much greater percentage (52 - 87%) of samples than the existing ruminant-specific assay (17%). These novel ruminant-specific assays show promise for microbial source tracking and merit further field testing and specificity evaluation. | en |
dc.description.sponsorship | ERTDI | en |
dc.format | application/pdf | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | Bifidobacteria | en |
dc.subject | Bacteroidales | en |
dc.subject | Microbial source tracking | en |
dc.subject | Fecal pollution | en |
dc.subject | PCR | en |
dc.subject.lcsh | Foodborne diseases | en |
dc.subject.lcsh | Microbial toxins | en |
dc.subject.lcsh | Pathogenic microorganisms -- Detection | en |
dc.subject.lcsh | Diagnostic microbiology. | en |
dc.title | Specificity and sensitivity evaluation of novel and existing Bacteroidales and Bifidobacteria-specific PCR assays on feces and sewage samples and their application for microbial source tracking in Ireland | en |
dc.type | Article | en |
dc.local.publishedsource | http://dx.doi.org/10.1016/j.watres.2009.08.050 | en |
dc.description.peer-reviewed | peer-reviewed | en |
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