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dc.contributor.authorUttam, Shikhar
dc.contributor.authorBista, Rajan K.
dc.contributor.authorStaton, Kevin
dc.contributor.authorAlexandrov, Sergey
dc.contributor.authorChoi, Serah
dc.contributor.authorBakkenist, Christopher J.
dc.contributor.authorHartman, Douglas J.
dc.contributor.authorBrand, Randall E.
dc.contributor.authorLiu, Yang
dc.date.accessioned2018-02-28T11:30:41Z
dc.date.available2018-02-28T11:30:41Z
dc.date.issued2013
dc.identifier.citationUttam, S,Bista, RK,Staton, K,Alexandrov, S,Choi, S,Bakkenist, CJ,Hartman, DJ,Brand, RE,Liu, Y (2013) 'Investigation of depth-resolved nanoscale structural changes in regulated cell proliferation and chromatin decondensation'. Biomedical Optics Express, 4 :596-613. doi: 10.1364/BOE.4.000596en_IE
dc.identifier.issn2156-7085
dc.identifier.urihttp://hdl.handle.net/10379/7176
dc.description.abstractWe present depth-resolved spatial-domain low-coherence quantitative phase microscopy, a simple approach that utilizes coherence gating to construct a depth-resolved structural feature vector quantifying sub-resolution axial structural changes at different optical depths within the sample. We show that this feature vector is independent of sample thickness variation, and identifies nanoscale structural changes in clinically prepared samples. We present numerical simulations and experimental validation to demonstrate the feasibility of the approach. We also perform experiments using unstained cells to investigate the nanoscale structural changes in regulated cell proliferation through cell cycle and chromatin decondensation induced by histone acetylation. (C) 2013 Optical Society of Americaen_IE
dc.description.sponsorshipThis study was supported in part by National Cancer Institute (CA164433 and CA148644), the Broad Medical Research Program of The Broad Foundation, Magee-Women’s Foundation, James F. Walsh Foundation and National Institute of Health through UL1RR024153 and UL1TR000005.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.relation.ispartofBiomedical Optics Expressen
dc.subjectDOMAIN PHASE MICROSCOPYen_IE
dc.subjectLIGHT-SCATTERINGen_IE
dc.subjectSPECTROSCOPIC MICROSCOPYen_IE
dc.subjectBIOLOGICAL CELLSen_IE
dc.subjectREFRACTIVE-INDEXen_IE
dc.subjectCANCERen_IE
dc.subjectDIAGNOSISen_IE
dc.subjectDYNAMICSen_IE
dc.subjectMARKERSen_IE
dc.titleInvestigation of depth-resolved nanoscale structural changes in regulated cell proliferation and chromatin decondensationen_IE
dc.typeArticleen_IE
dc.date.updated2016-02-02T10:19:42Z
dc.identifier.doi10.1364/BOE.4.000596
dc.local.publishedsourcehttps://doi.org/10.1364/BOE.4.000596en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funderNational Cancer Instituteen_IE
dc.internal.rssid4339889
dc.local.contactSergey Alexandrov, School Of Physics, Nui Galway. Email: sergey.alexandrov@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionPUBLISHED
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