Cartilage repair in a rabbit model: Development of a novel subchondral defect and assessment of early cartilage repair using a rabbit mesenchymal stem cell seeded scaffold
MetadataShow full item record
This item's downloads: 11 (view details)
Purpose: To develop a subchondral defect in a rabbit model and assess cartilage repair using a mesenchymal stem cell-seeded scaffold. Materials and Methods: To achieve this goal, a bovine explant model and two pilot rabbit studies were conducted. Initial work using chondral explant tissue harvested from stifle joints of 12-month-old cattle sourced from a local abattoir, followed by a pilot rabbit study demonstrated that a chondral defect was an unsuitable model for the scaffold being studied. Assessment of a novel reproducible technique for subchondral defect creation was conducted using a rabbit model in accordance with University guidelines and ethical approval with 4 test groups, empty 3mm x1mm defect (n=6), empty 3mm x3mm defect (n=6), press-fit scaffold in 3mm x1mm defect (n=6), press-fit cell-seeded scaffold in 3mm x1mm defect (n=6). l. In brief, skeletally mature rabbits, weighing more than 3kg were anesthetized and bilateral defects were created on the medial femoral condyle using an electrical drill with a previously sterilized 2.8mm drill bit covered with a sterile depth stop. The walls of the defect were finished with a dental curette, and the scaffold press-fit into place. Defects was assessed histologically after 28 days implantation. Results: The subchondral defects could be created with the same reproducibility as osteochondral defects and tissue repair was achieved in both cell free and cell-seeded scaffolds. Conclusion: A reproducible subchondral defect was successfully created in a rabbit model to assess cartilage repair using a cell-seeded scaffold. Early cartilage repair was achieved in both cell free and cell-seeded scaffolds, with improved repair seen in the cell free scaffold group
This item is available under the Attribution-NonCommercial-NoDerivs 3.0 Ireland. No item may be reproduced for commercial purposes. Please refer to the publisher's URL where this is made available, or to notes contained in the item itself. Other terms may apply.
The following license files are associated with this item: