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dc.contributor.advisorRea, Stephen
dc.contributor.authorMeller, Anna
dc.date.accessioned2018-01-22T09:22:21Z
dc.date.issued2018-01-14
dc.identifier.urihttp://hdl.handle.net/10379/7102
dc.description.abstractWhile the components of the MSL (Male Specific Lethal) complex in Drosophila are well studied and known to play a critical role in dosage compensation, there is little known about the function of its evolutionary conserved human orthologues. To better understand how the MSL complex is involved in cellular processes this study focuses on two subunits, MSL2 and MSL1. To decipher the function of human MSL2 and MSL1 within the MSL complex a loss-of-function approach was chosen using the CRISPR/Cas9 genome editing method. Based on previous findings further characterization of these proteins in the DNA damage response was performed. Using immunofluorescence microscopy perturbed 53BP1 nuclear body formation was observed in cells lacking either MSL2 or MSL1 and a possible role in replication related DNA damage was studied as well. Using a modified proximity biotin ligation based BioID approach novel interactors and possible substrates of the E3 ubiquitin ligase MSL2 were also identified.en_IE
dc.subjectMSL2en_IE
dc.subjectMSL1en_IE
dc.subjectDNA damage responseen_IE
dc.subjectReplicationen_IE
dc.subjectBioIDen_IE
dc.subjectUbiquitylationen_IE
dc.subjectBiochemistryen_IE
dc.subjectNatural scienceen_IE
dc.titleDeciphering the role of human MSL2 and MSL1 within and beyond the MSL complexen_IE
dc.typeThesisen_IE
dc.local.noteThis work describes investigations made to further characterise and understand the functions of two human proteins, MSL2 and MSL1. The main interest of the study was to further characterise their role in response to breaks occurring in the DNA and to identify novel interactors and possible substrates of MSL2.en_IE
dc.description.embargo2022-01-14
dc.local.finalYesen_IE
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