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dc.contributor.authorConnelly, Stephanie
dc.contributor.authorShin, Seung Gu
dc.contributor.authorDillon, Robert J.
dc.contributor.authorIjaz, Umer Zeeshan
dc.contributor.authorQuince, Christopher
dc.contributor.authorSloan, William
dc.contributor.authorCollins, Gavin
dc.date.accessioned2017-09-22T11:43:27Z
dc.date.available2017-09-22T11:43:27Z
dc.date.issued2017
dc.identifier.citationConnelly, Stephanie, Shin, Seung G., Dillon, Robert J., Ijaz, Umer Z., Quince, Christopher, Sloan, William T., & Collins, Gavin. (2017). Bioreactor Scalability: Laboratory-Scale Bioreactor Design Influences Performance, Ecology, and Community Physiology in Expanded Granular Sludge Bed Bioreactors. Frontiers in Microbiology, 8(664). doi: 10.3389/fmicb.2017.00664en_IE
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10379/6827
dc.description.abstractStudies investigating the feasibility of new, or improved, biotechnologies, such as wastewater treatment digesters, inevitably start with laboratory-scale trials. However, it is rarely determined whether laboratory-scale results reflect full-scale performance or microbial ecology. The Expanded Granular Sludge Bed (EGSB) bioreactor, which is a high-rate anaerobic digester configuration, was used as a model to address that knowledge gap in this study. Two laboratory-scale idealizations of the EGSB-a one-dimensional and a three-dimensional scale-down of a full-scale design-were built and operated in triplicate under near-identical conditions to a full-scale EGSB. The laboratory-scale bioreactors were seeded using biomass obtained from the full-scale bioreactor, and, spent water from the distillation of whisky from maize was applied as substrate at both scales. Over 70 days, bioreactor performance, microbial ecology, and microbial community physiology were monitored at various depths in the sludge-beds using 16S rRNA gene sequencing (V4 region), specific methanogenic activity (SMA) assays, and a range of physical and chemicalmonitoringmethods. SMA assays indicated dominance of the hydrogenotrophic pathway at full-scale whilst a more balanced activity profile developed during the laboratory-scale trials. At each scale, Methanobacterium was the dominant methanogenic genus present. Bioreactor performance overall was better at laboratory-scale than full-scale. We observed that bioreactor design at laboratory-scale significantly influenced spatial distribution of microbial community physiology and taxonomy in the bioreactor sludge-bed, with 1-D bioreactor types promoting stratification of each. In the 1-D laboratory bioreactors, increased abundance of Firmicutes was associated with both granule position in the sludge bed and increased activity against acetate and ethanol as substrates. We further observed that stratification in the sludge-bed in 1-D laboratory-scale bioreactors was associated with increased richness in the underlying microbial community at species (OTU) level and improved overall performance.en_IE
dc.description.sponsorshipThe authors thank Carole Jude and North British Distillery for providing support throughout the sampling of the full-scale bioreactor, and for making available performance data from full-scale. The study was funded by the Engineering and Physical Sciences Research Council, UK (EP/J00538X/1; EP/K038885/1). GC was supported by a European Research Council Starting Grant (3C-BIOTECH 261330). UI was funded by NERC IRF NE/L011956/1. CQ was funded by an MRC fellowship MR/M50161X/1 as part of the CLoud Infrastructure for Microbial Genomics (CLIMB) consortium MR/L015080/1.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherFrontiers Mediaen_IE
dc.relation.ispartofFrontiers In Microbiologyen
dc.subject16S rRNA geneen_IE
dc.subjectanaerobic digestionen_IE
dc.subjectEGSBen_IE
dc.subjectIllumina MiSeqen_IE
dc.subjectlaboratory-scaleen_IE
dc.subjectfull-scaleen_IE
dc.subjectindustrial wastewateren_IE
dc.subjectspecific methanogenic activityen_IE
dc.subjectEGSBen_IE
dc.subjectAnaerobic digestionen_IE
dc.subjectLaboratory-scaleen_IE
dc.subjectDynamicsen_IE
dc.subjectDiversityen_IE
dc.subjectArchaelen_IE
dc.subjectDigestionen_IE
dc.subjectSequencesen_IE
dc.subjectEGSBen_IE
dc.subjectBacterialen_IE
dc.subjectBatch reactoren_IE
dc.subjectWaste water treatmenten_IE
dc.subjectAnaerobic biological treatmenten_IE
dc.titleBioreactor scalability: laboratory-scale bioreactor design influences performance, ecology, and community physiology in expanded granular sludge bed bioreactorsen_IE
dc.typeArticleen_IE
dc.date.updated2017-09-21T12:57:17Z
dc.identifier.doi10.3389/fmicb.2017.00664
dc.local.publishedsourcehttps://doi.org/10.3389/fmicb.2017.00664en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid12668343
dc.local.contactGavin Collins, Room 206 , Microbiology, School Of Natural Sciences, Nui Galway. 3163 Email: gavin.collins@nuigalway.ie
dc.local.copyrightcheckedYes Open Access
dc.local.versionPUBLISHED
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