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dc.contributor.authorDomozych, David S.
dc.contributor.authorSørensen, Iben
dc.contributor.authorPopper, Zoë A.
dc.contributor.authorOchs, Julie
dc.contributor.authorAndreas, Amanda
dc.contributor.authorFangel, Jonatan U.
dc.contributor.authorPielach, Anna
dc.contributor.authorSacks, Carly
dc.contributor.authorBrechka, Hannah
dc.contributor.authorRuisi-Besares, Pia
dc.contributor.authorWillats, William G.T.
dc.contributor.authorRose, Jocelyn K.C.
dc.date.accessioned2017-04-20T13:47:40Z
dc.date.available2017-04-20T13:47:40Z
dc.date.issued2014-03-20
dc.identifier.citationDomozych, DS,Sorensen, I,Popper, ZA,Ochs, J,Andreas, A,Fangel, JU,Pielach, A,Sacks, C,Brechka, H,Ruisi-Besares, P,Willats, WGT,Rose, JKC (2014) 'Pectin Metabolism and Assembly in the Cell Wall of the Charophyte Green Alga Penium margaritaceum'. Plant Physiol, 165 :105-118, DOI:10.1104/pp.114.236257en_IE
dc.identifier.issn1532-2548
dc.identifier.urihttp://hdl.handle.net/10379/6468
dc.description.abstractThe pectin polymer homogalacturonan (HG) is a major component of land plant cell walls and is especially abundant in the middle lamella. Current models suggest that HG is deposited into the wall as a highly methylesterified polymer, demethylesterified by pectin methylesterase enzymes and cross-linked by calcium ions to form a gel. However, this idea is based largely on indirect evidence and in vitro studies. We took advantage of the wall architecture of the unicellular alga Penium margaritaceum, which forms an elaborate calcium cross-linked HG-rich lattice on its cell surface, to test this model and other aspects of pectin dynamics. Studies of live cells and microscopic imaging of wall domains confirmed that the degree of methylesterification and sufficient levels of calcium are critical for lattice formation in vivo. Pectinase treatments of live cells and immunological studies suggested the presence of another class of pectin polymer, rhamnogalacturonan I, and indicated its colocalization and structural association with HG. Carbohydrate microarray analysis of the walls of P. margaritaceum, Physcomitrella patens, and Arabidopsis (Arabidopsis thaliana) further suggested the conservation of pectin organization and interpolymer associations in the walls of green plants. The individual constituent HG polymers also have a similar size and branched structure to those of embryophytes. The HG-rich lattice of P. margaritaceum, a member of the charophyte green algae, the immediate ancestors of land plants, was shown to be important for cell adhesion. Therefore, the calcium-HG gel at the cell surface may represent an early evolutionary innovation that paved the way for an adhesive middle lamella in multicellular land plants.en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherAmerican Society of Plant Biologistsen_IE
dc.relation.ispartofPlant Physiolen
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectSugar beet rooten_IE
dc.subjectMonoclonal antibodiesen_IE
dc.subjectRipening mutanten_IE
dc.subjectMiddle lamellaen_IE
dc.subjectSide chainsen_IE
dc.subjectAdhesionen_IE
dc.subjectCalciumen_IE
dc.subjectHomogalacturonanen_IE
dc.subjectArabidopsisen_IE
dc.subjectPolysaccharidesen_IE
dc.titlePectin metabolism and assembly in the cell wall of the charophyte green alga penium margaritaceumen_IE
dc.typeArticleen_IE
dc.date.updated2017-04-10T12:00:08Z
dc.identifier.doi10.1104/pp.114.236257
dc.local.publishedsourcehttp://dx.doi.org/10.1104/pp.114.236257en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid6513652
dc.local.contactZoe Adelaide Popper, Department Of Botany, Nuig. 5431 Email: zoe.popper@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionPUBLISHED
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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland