dc.contributor.author | Hortigüela, María J. | |
dc.contributor.author | Lucie Aumailley, Lucie | |
dc.contributor.author | Srivastava, Akshay | |
dc.contributor.author | Cunningham, Clare | |
dc.contributor.author | Anandakumar, Soshee | |
dc.contributor.author | Robin, Sylvain | |
dc.contributor.author | Pandit, Abhay | |
dc.contributor.author | Hu, Xuejun | |
dc.contributor.author | Wall, Gerard | |
dc.date.accessioned | 2016-12-14T15:21:59Z | |
dc.date.available | 2016-12-14T15:21:59Z | |
dc.date.issued | 2015-08-18 | |
dc.identifier.citation | Hortigüela, M. J., Aumailley, L., Srivastava, A., Cunningham, C., Anandakumar, S., Robin, S., Pandit, A., Hu, X., and Wall, J. G. (2015) Engineering recombinant antibodies for polymer biofunctionalization. Polym. Adv. Technol., 26: 1394–1401. doi: 10.1002/pat.3619. | en_IE |
dc.identifier.issn | 1099-1581 | |
dc.identifier.uri | http://hdl.handle.net/10379/6246 | |
dc.description.abstract | The attachment of recognition elements such as antibody fragments to polymeric substrates can be used to mediate cell- or protein-specific interactions. In this work, single-chain Fv (scFv) antibody fragments were isolated against two cell types of interest and expressed in an Escherichia coli expression platform. The scFvs were engineered at their C-terminus to incorporate a cysteine-containing linker, for reaction with maleimide-linked polymers, or a heptasaccharide glycan for complexation with surface amine moieties. Antigen binding of the modified scFvs was unchanged, and expression yields of the glyco-engineered scFvs were similar to the unmodified molecules, while cys-tagged scFv yields varied between scFv variants. Targeted immobilization of the scFvs via either modification resulted in three-to five-fold higher binding of ligands over adsorbed molecules. The study demonstrates a simple and efficient antibody engineering and modification approach for effective targeted immobilization on polymeric substrates. Copyright (C) 2015 John Wiley & Sons, Ltd. | en_IE |
dc.description.sponsorship | This work was supported by Irish Research Council (IRC) grantPD/2010/1689 (MJH), Science Foundation Ireland Grant 07/SRC/B1163 (CC, AS), Enterprise Ireland Science and TechnologyAgency Grant PC/2007/021 (SR) and European Union “EPICstent”Project Grant FP7-PEOPLE-2012-IAPP-324514 (SA). | en_IE |
dc.format | application/pdf | en_IE |
dc.language.iso | en | en_IE |
dc.publisher | Wiley | en_IE |
dc.relation.ispartof | Polymers For Advanced Technologies | en |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | Biomedical engineering | en_IE |
dc.subject | Recombinant protein | en_IE |
dc.subject | Antibody fragment | en_IE |
dc.subject | Immobilization | en_IE |
dc.subject | Biofunctionalization | en_IE |
dc.subject | Escherichia coli | en_IE |
dc.subject | Biomedical appliocations | en_IE |
dc.subject | Domoic acid | en_IE |
dc.subject | Affinity maturation | en_IE |
dc.subject | Monoclonal antibody | en_IE |
dc.subject | Variable domains | en_IE |
dc.subject | In vitro | en_IE |
dc.subject | Fragment | en_IE |
dc.subject | Binding | en_IE |
dc.title | Engineering recombinant antibodies for polymer biofunctionalization | en_IE |
dc.type | Article | en_IE |
dc.date.updated | 2016-12-14T15:05:47Z | |
dc.identifier.doi | 10.1002/pat.3619 | |
dc.local.publishedsource | http:dx.doi.org/10.1002/pat.3619 | en_IE |
dc.description.peer-reviewed | peer-reviewed | |
dc.contributor.funder | |~| | |
dc.internal.rssid | 10558974 | |
dc.local.contact | Abhay Shashikant Pandit, Curam, Biomedical Sciences, Nui Galway. 2758 Email: abhay.pandit@oegaillimh.ie | |
dc.local.copyrightchecked | No | |
dc.local.version | ACCEPTED | |
nui.item.downloads | 427 | |