Browsing Biomedical Engineering (Scholarly Articles) by Author "McNamara, Laoise M."
Now showing items 1-20 of 21
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Altered mechanical environment of bone cells in an animal model of short- and long-term osteoporosis
Verbruggen, Stefaan W.; McGarrigle, Myles J.; Haugh, Matthew G.; Voisin, Muriel C.; McNamara, Laoise M. (Biophysical Society, 2015-04-07)Alterations in bone tissue composition during osteoporosis likely disrupt the mechanical environment of bone cells and may thereby initiate a mechanobiological response. It has proved challenging to characterize the ... -
Are all osteocytes equal? Multiscale modelling of cortical bone to characterise the mechanical stimulation of osteocytes
Vaughan, Ted J.; Verbruggen, Stefaan W.; McNamara, Laoise M. (Wiley, 2013-12-03)Bone continuously adapts its internal structure to accommodate the functional demands of its mechanical environment. This process is orchestrated by a network of mechanosensitive osteocytes that respond to external ... -
Differential β3 and β1 integrin expression in bone marrow and cortical bone of estrogen deficient rats
Voisin, Muriel C.; McNamara, Laoise M. (Wiley, 2015-08-12)Integrin-based (beta(3)) attachments to the extracellular matrix (ECM) on osteocyte cell processes have recently been proposed to play an important role in facilitating osteocyte mechanosensation. However, it is not yet ... -
The effect of substrate stiffness, thickness, and cross-linking density on osteogenic cell behavior
Mullen, Conleth A.; Vaughan, Ted J.; Billiar, Kristen L.; McNamara, Laoise M. (Biophysical Society, 2015-04-07)Osteogenic cells respond to mechanical changes in their environment by altering their spread area, morphology, and gene expression profile. In particular, the bulk modulus of the substrate, as well as its microstructure ... -
Effects of in vitro endochondral priming and pre-vascularisation of human MSC cellular aggregates in vivo
Freeman, Fiona E.; Allen, Ashley B.; Stevens, Hazel Y.; Guldberg, Robert E.; McNamara, Laoise M. (2015)Introduction: During endochondral ossification, both the production of a cartilage template and the subsequent vascularisation of that template are essential precursors to bone tissue formation. Recent studies have found ... -
Estrogen deficiency impairs integrin αvβ3-mediated mechanosensation by osteocytes and alters osteoclastogenic paracrine signalling
Geoghegan, Ivor P.; Hoey, David A.; McNamara, Laoise M. (Nature Research (part of Springer Nature), 2019-03-15)The integrin alpha(v)beta(3) has been shown to play an important role in osteocyte mechanotransduction. It has been reported that there are fewer beta(3) integrin-containing cells in osteoporotic bone cells. Osteocytes ... -
Fluid flow in the osteocyte mechanical environment: a fluid-structure interaction approach
Verbruggen, Stefaan W.; Vaughan, Ted J.; McNamara, Laoise M. (Springer Verlag, 2013-04-09)Osteocytes are believed to be the primary sensor of mechanical stimuli in bone, which orchestrate osteoblasts and osteoclasts to adapt bone structure and composition to meet physiological loading demands. Experimental ... -
An in vitro bone tissue regeneration strategy combining chondrogenic and vascular priming enhances the mineralization potential of mesenchymal stem cells In vitro while also allowing for vessel formation
Freeman, Fiona E.; Haugh, Matthew G.; McNamara, Laoise M. (Mary Ann Liebert, 2015-03-03)Chondrogenic priming (CP) of mesenchymal stem cells (MSCs) and coculture of MSCs with human umbilical vein endothelial stem cells (HUVECs) both have been shown to significantly increase the potential for MSCs to undergo ... -
Investigation of the optimal timing for chondrogenic priming of MSCs to enhance osteogenic differentiation in vitro as a bone tissue engineering strategy
Freeman, Fiona E.; Haugh, Matthew G.; McNamara, Laoise M. (Wiley, 2013-08-07)Recent in vitro tissue engineering approaches have shown that chondrogenic priming of human bone marrow mesenchymal stem cells (MSCs) can have a positive effect on osteogenesis in vivo. However, whether chondrogenic ... -
Mechanical stimulation of bone marrow in situ induces bone formation in trabecular explants
Birmingham, Evelyn; Kreipke, T. C.; Dolan, E.B.; Coughlin, T.R.; Owens, Peter; McNamara, Laoise M.; Niebur, G.L.; McHugh, Peter E. (Springer Verlag, 2014-10-04)Abstract Low magnitude high frequency (LMHF) loading has been shown to have an anabolic effect on trabecular bone in vivo. However, the precise mechanical signal imposed on the bone marrow cells by LMHF loading, which ... -
Mechanisms of osteocyte stimulation in osteoporosis
Verbruggen, Stefaan W.; Vaughan, Ted J.; McNamara, Laoise M. (Elsevier, 2016-05-10)Experimental studies have shown that primary osteoporosis' caused by oestrogen deficiency results in localised alterations in bone tissue properties and mineral composition. Additionally, changes to the lacunar-canalicular ... -
Multiscale fluid-structure interaction modelling to determine the mechanical stimulation of bone cells in a tissue engineered scaffold
Zhao, Feihu; Vaughan, Ted J.; McNamara, Laoise M. (2015-04)Recent studies have shown that mechanical stimulation, by means of flow perfusion and mechanical compression (or stretching), enhances osteogenic differentiation of mesenchymal stem cells and bone cells within biomaterial ... -
Osteocyte differentiation and the formation of an interconnected cellular network in vitro
Mc Garrigle, M.J.; Mullen, Conleth A.; Haugh, Matthew G.; Voisin, Muriel C.; McNamara, Laoise M. (AO Research Institute Davos, 2016-05-26)Extracellular matrix (ECM) stiffness and cell density can regulate osteoblast differentiation in two dimensional environments. However, it is not yet known how osteoblast-osteocyte differentiation is regulated within a 3D ... -
Osteocyte differentiation is regulated by extracellular matrix stiffness and intercellular separation
Mullen, Conleth A.; Haugh, Matthew G.; Schaffler, M. B.; Majeska, R. J.; McNamara, Laoise M. (Elsevier, 2013-07-18)Osteocytes are terminally differentiated bone cells, derived from osteoblasts, which are vital for regulation of bone formation and resorption. ECM stiffness and cell seeding density have been shown to regulate osteoblast ... -
Osteogenic differentiation of mesenchymal stem cells is regulated by osteocyte and osteoblast cells in a simplified bone niche
Birmingham, Evelyn; Niebur, G.L.; McHugh, Peter E.; Shaw, G.; Barry, F.P.; McNamara, Laoise M. (AO Research Institute Davos, 2012)Mesenchymal stem cells (MSCs) within their native environment of the stem cell niche in bone receive biochemical stimuli from surrounding cells. These stimuli likely influence how MSCs differentiate to become bone precursors. ... -
Primary cilia exist in a small fraction of cells in trabecular bone and marrow
Coughlin, Thomas R.; Voisin, Muriel C.; Schaffler, Mitchell B.; Niebur, Glen L.; McNamara, Laoise M. (Springer, 2014-11-15)Primary cilia are potent mechanical and chemical sensory organelles in cells of bone lineage in tissue culture. Cell culture experiments suggest that primary cilia sense fluid flow and this stimulus is translated through ... -
Quantification of fluid shear stress in bone tissue engineering scaffolds with spherical and cubical pore architectures
Zhao, Feihu; Vaughan, Ted J.; McNamara, Laoise M. (Springer Verlag, 2016-06)Recent studies have shown that mechanical stimulation, in the form of fluid perfusion and mechanical compression, can enhance osteogenic differentiation of mesenchymal stem cells and bone cells within tissue engineering ... -
The role of integrin αVβ3 in osteocyte mechanotransduction
Haugh, Matthew G.; Vaughan, Ted J.; McNamara, Laoise M. (Elsevier, 2014-11-10)Recent in vivo studies have proposed that integrin alpha(v)beta(3) attachments between osteocyte cell processes and the extracellular matrix may facilitate mechanosensation in bone. However the role of these attachments ... -
Strain amplification in bone mechanobiology: a computational investigation of the in vivo mechanics of osteocytes
Verbruggen, Stefaan W.; Vaughan, Ted J.; McNamara, Laoise M. (The Royal Society Publishing, 2012-06-06)The osteocyte is believed to act as the main sensor of mechanical stimulus in bone, controlling signalling for bone growth and resorption in response to changes in the mechanical demands placed on our bones throughout life. ... -
Thermally induced osteocyte damage initiates a remodelling signaling cascade
Dolan, Eimear B.; Haugh, Margaret; Voisin, Muriel C.; Tallon, David; McNamara, Laoise M. (Public Library of Science, 2015-03-18)Thermal elevations experienced by bone during orthopaedic procedures, such as cutting and drilling, exothermal reactions from bone cement, and thermal therapies such as tumor ablation, can result in thermal damage leading ...