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dc.contributor.authorMallinson, David
dc.contributor.authorCheung, David L.
dc.contributor.authorSimionesie, Dorin
dc.contributor.authorMullen, Alexander B.
dc.contributor.authorZhang, Zhenyu J.
dc.contributor.authorLamprou, Dimitrios A.
dc.date.accessioned2016-11-01T20:29:01Z
dc.date.issued2016-10-24
dc.identifier.citationMallinson, D., Cheung, D. L., Simionesie, D., Mullen, A. B., Zhang, Z. J. and Lamprou, D. A. (2016), Experimental and computational examination of anastellin (FnIII1c)-polymer interactions. J. Biomed. Mater. Res.. Accepted Author Manuscript. doi:10.1002/jbm.a.35949en_IE
dc.identifier.issn1552-4965)
dc.identifier.urihttp://hdl.handle.net/10379/6115
dc.description.abstractUsing a combination of experimental and computational approaches, the interaction between anastellin, a recombinant fragment of fibronectin, and representative biomaterial surfaces has been examined. Anastellin and superfibronectin, have been seen to exhibit anti-angiogenic properties and other properties that may make it suitable for consideration for incorporation into biomaterials. The molecular interaction was directly quantified by atomic force microscope (AFM) based force spectroscopy, complemented by adsorption measurements using quartz crystal microbalance (QCM). By AFM, it was found that the anastellin molecule facilitates a stronger adhesion on polyurethane films (72.0 pN nm-1 ) than on poly (methyl methacrylate) films (68.6 pN nm-1 ). However, this is not consistent with the QCM adsorption measurements which shows no significant difference. Molecular dynamics simulations of the behaviour of anastellin on polyurethane in aqueous solution were performed to rationalise the experimental data, and show that anastellin is capable of rapid adsorption to PU while its secondary structure is stable upon adsorption in water.en_IE
dc.description.sponsorshipThe authors would like to acknowledge the UK Engineering & Physical Sciences Research Council (EPSRC) and the University of Strathclyde for the studentship to David Mallinson, and the Bridging the gap (BTG) project at the University of Strathclyde for funding this work. Computational facilities were provided by the Archie-West HPC facility (EPSRC grant no. EP/K000586/1), and SFI/HEA Irish Centre for High-End Computing (ICHEC) and access to AFM and contact angle measurements was provided by the EPSRC Centre in Continuous Manufacturing and Crystallisation (CMAC).en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherWileyen_IE
dc.relation.ispartofJournal Of Biomedical Materials Research - Part Aen
dc.subjectChemistryen_IE
dc.subjectAtomic force microscopyen_IE
dc.subjectMolecular dynamicsen_IE
dc.subjectPolyurethaneen_IE
dc.subjectPoly (methyl methacrylate)en_IE
dc.subjectFibronectin.en_IE
dc.titleExperimental and computational examination of anastellin (FnIII1c)-polymer interactionsen_IE
dc.typeArticleen_IE
dc.date.updated2016-10-27T13:26:12Z
dc.identifier.doi10.1002/jbm.a.35949
dc.local.publishedsourcehttp://dx.doi.org/10.1002/jbm.a.35949en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|1267880|~|1267883|~|
dc.description.embargo2017-10-24
dc.internal.rssid11584424
dc.local.contactDavid Cheung, School Of Chemistry, Nuig. Email: david.cheung@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
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