Experimental and computational examination of anastellin (FnIII1c)-polymer interactions
Date
2016-10-24Author
Mallinson, David
Cheung, David L.
Simionesie, Dorin
Mullen, Alexander B.
Zhang, Zhenyu J.
Lamprou, Dimitrios A.
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Mallinson, D., Cheung, D. L., Simionesie, D., Mullen, A. B., Zhang, Z. J. and Lamprou, D. A. (2016), Experimental and computational examination of anastellin (FnIII1c)-polymer interactions. J. Biomed. Mater. Res.. Accepted Author Manuscript. doi:10.1002/jbm.a.35949
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Abstract
Using a combination of experimental and computational approaches, the
interaction between anastellin, a recombinant fragment of fibronectin, and representative
biomaterial surfaces has been examined. Anastellin and superfibronectin, have been seen to
exhibit anti-angiogenic properties and other properties that may make it suitable for
consideration for incorporation into biomaterials. The molecular interaction was directly
quantified by atomic force microscope (AFM) based force spectroscopy, complemented by
adsorption measurements using quartz crystal microbalance (QCM). By AFM, it was found
that the anastellin molecule facilitates a stronger adhesion on polyurethane films (72.0 pN
nm-1
) than on poly (methyl methacrylate) films (68.6 pN nm-1
). However, this is not
consistent with the QCM adsorption measurements which shows no significant difference.
Molecular dynamics simulations of the behaviour of anastellin on polyurethane in aqueous
solution were performed to rationalise the experimental data, and show that anastellin is
capable of rapid adsorption to PU while its secondary structure is stable upon adsorption in
water.