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dc.contributor.authorRonan, William
dc.contributor.authorMcGarry, J. Patrick
dc.identifier.citationRonan, William, Deshpande, Vikram S., McMeeking, Robert M., & McGarry, J. Patrick. (2012). Numerical investigation of the active role of the actin cytoskeleton in the compression resistance of cells. Journal of the Mechanical Behavior of Biomedical Materials, 14, 143-157. doi:
dc.descriptionJournal articleen_IE
dc.description.abstractNumerous in-vitro studies have established that cells react to their physical environment and to applied mechanical loading. However, the mechanisms underlying such phenomena are poorly understood. Previous modelling of cell compression considered the cell as a passive homogenous material, requiring an artificial increase in the stiffness of spread cells to replicate experimentally measured forces. In this study, we implement a fully 3D active constitutive formulation that predicts the distribution, remodelling, and contractile behaviour of the cytoskeleton. Simulations reveal that polarised and axisymmetric spread cells contain stress fibres which form dominant bundles that are stretched during compression. These dominant fibres exert tension; causing an increase in computed compression forces compared to round cells. In contrast, fewer stress fibres are computed for round cells and a lower resistance to compression is predicted. The effect of different levels of cellular contractility associated with different cell phenotypes is also investigated. Highly contractile cells form more dominant circumferential stress fibres and hence provide greater resistance to compression. Computed predictions correlate strongly with published experimentally observed trends of compression resistance as a function of cellular contractility and offer an insight into the link between cell geometry, stress fibre distribution and contractility, and cell deformability. Importantly, it is possible to capture the behaviour of both round and spread cells using a given, unchanged set of material parameters for each cell type. Finally, it is demonstrated that stress distributions in the cell cytoplasm and nucleus computed using the active formulation differ significantly from those computed using passive material models.en_IE
dc.publisherElsevier ScienceDirecten_IE
dc.relation.ispartofJournal Of The Mechanical Behavior Of Biomedical Materialsen
dc.subjectStress fibreen_IE
dc.subjectActive contractilityen_IE
dc.subjectConstitutive formulationen_IE
dc.subjectCell compressionen_IE
dc.subjectSingle attached cellsen_IE
dc.subjectStress fiberen_IE
dc.subjectMechanical propertiesen_IE
dc.subjectMicropipette aspirationen_IE
dc.subjectViscoelastic propertiesen_IE
dc.subjectEndothelial cellsen_IE
dc.subjectMatrix elasticityen_IE
dc.subjectIn vitroen_IE
dc.titleNumerical investigation of the active role of the actin cytoskeleton in the compression resistance of cellsen_IE
dc.identifier.doiDOI 10.1016/j.jmbbm.2012.05.016
dc.local.contactWilliam Ronan, Mechanical Engineering, School Of Engineering, Nui Galway. Email:

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