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dc.contributor.authorDowling, Enda P.
dc.contributor.authorRonan, William
dc.contributor.authorOfek, Gidon
dc.contributor.authorDeshpande, Vikram S.
dc.contributor.authorMcMeeking, Robert M.
dc.contributor.authorAthanasiou, Kyriacos A.
dc.identifier.citationDowling, Enda P., Ronan, William, Ofek, Gidon, Deshpande, Vikram S., McMeeking, Robert M., Athanasiou, Kyriacos A., & McGarry, J. Patrick. (2012). The effect of remodelling and contractility of the actin cytoskeleton on the shear resistance of single cells: a computational and experimental investigation. Journal of The Royal Society Interface, 9(77), 3469-3479. doi: 10.1098/rsif.2012.0428en_IE
dc.description.abstractThe biomechanisms that govern the response of chondrocytes to mechanical stimuli are poorly understood. In this study, a series of in vitro tests are performed, in which single chondrocytes are subjected to shear deformation by a horizontally moving probe. Dramatically different probe force-indentation curves are obtained for untreated cells and for cells in which the actin cytoskeleton has been disrupted. Untreated cells exhibit a rapid increase in force upon probe contact followed by yielding behaviour. Cells in which the contractile actin cytoskeleton was removed exhibit a linear force-indentation response. In order to investigate the mechanisms underlying this behaviour, a three-dimensional active modelling framework incorporating stress fibre (SF) remodelling and contractility is used to simulate the in vitro tests. Simulations reveal that the characteristic force-indentation curve observed for untreated chondrocytes occurs as a result of two factors: (i) yielding of SFs due to stretching of the cytoplasm near the probe and (ii) dissociation of SFs due to reduced cytoplasm tension at the front of the cell. In contrast, a passive hyperelastic model predicts a linear force-indentation curve similar to that observed for cells in which the actin cytoskeleton has been disrupted. This combined modelling-experimental study offers a novel insight into the role of the active contractility and remodelling of the actin cytoskeleton in the response of chondrocytes to mechanical loading.en_IE
dc.description.sponsorshipIrish Research Council for Science, Engineering and Technology (IRCSET) postgraduate scholarship under the EMBARK initiative, and by the Science Foundation Ireland Research Frontiers Programme (SFI-RFP/ENM1726) and Short Term Travel Fellowship (SFI-STTF)en_IE
dc.publisherThe Royal Society Publishingen_IE
dc.relation.ispartofJournal Of The Royal Society Interfaceen
dc.subjectCell mechanicsen_IE
dc.subjectActin cytoskeletonen_IE
dc.subjectCell contractilityen_IE
dc.subjectFinite elementen_IE
dc.subjectIn vitro shearen_IE
dc.subjectGene expressionen_IE
dc.subjectMicropipette aspirationen_IE
dc.subjectChondrocyte cytoskeletonen_IE
dc.subjectViscoelastic propertiesen_IE
dc.subjectMechanical compressionen_IE
dc.subjectArticular cartilageen_IE
dc.subjectAgarose constructsen_IE
dc.subjectConfocal analysisen_IE
dc.subjectIn vitroen_IE
dc.subjectMechanical engineeringen_IE
dc.subjectBiomedical engineeringen_IE
dc.titleThe effect of remodelling and contractility of the actin cytoskeleton on the shear resistance of single cells: a computational and experimental investigationen_IE
dc.local.contactWilliam Ronan, Mechanical Engineering, School Of Engineering, Nui Galway. Email:

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