dc.contributor.author | Dowling, Enda P. | |
dc.contributor.author | Ronan, William | |
dc.contributor.author | Ofek, Gidon | |
dc.contributor.author | Deshpande, Vikram S. | |
dc.contributor.author | McMeeking, Robert M. | |
dc.contributor.author | Athanasiou, Kyriacos A. | |
dc.date.accessioned | 2016-08-10T07:47:21Z | |
dc.date.available | 2016-08-10T07:47:21Z | |
dc.date.issued | 2012-07-18 | |
dc.identifier.citation | Dowling, Enda P., Ronan, William, Ofek, Gidon, Deshpande, Vikram S., McMeeking, Robert M., Athanasiou, Kyriacos A., & McGarry, J. Patrick. (2012). The effect of remodelling and contractility of the actin cytoskeleton on the shear resistance of single cells: a computational and experimental investigation. Journal of The Royal Society Interface, 9(77), 3469-3479. doi: 10.1098/rsif.2012.0428 | en_IE |
dc.identifier.issn | 1742-5662 | |
dc.identifier.uri | http://hdl.handle.net/10379/5939 | |
dc.description.abstract | The biomechanisms that govern the response of chondrocytes to mechanical stimuli are poorly understood. In this study, a series of in vitro tests are performed, in which single chondrocytes are subjected to shear deformation by a horizontally moving probe. Dramatically different probe force-indentation curves are obtained for untreated cells and for cells in which the actin cytoskeleton has been disrupted. Untreated cells exhibit a rapid increase in force upon probe contact followed by yielding behaviour. Cells in which the contractile actin cytoskeleton was removed exhibit a linear force-indentation response. In order to investigate the mechanisms underlying this behaviour, a three-dimensional active modelling framework incorporating stress fibre (SF) remodelling and contractility is used to simulate the in vitro tests. Simulations reveal that the characteristic force-indentation curve observed for untreated chondrocytes occurs as a result of two factors: (i) yielding of SFs due to stretching of the cytoplasm near the probe and (ii) dissociation of SFs due to reduced cytoplasm tension at the front of the cell. In contrast, a passive hyperelastic model predicts a linear force-indentation curve similar to that observed for cells in which the actin cytoskeleton has been disrupted. This combined modelling-experimental study offers a novel insight into the role of the active contractility and remodelling of the actin cytoskeleton in the response of chondrocytes to mechanical loading. | en_IE |
dc.description.sponsorship | Irish Research Council for Science, Engineering and Technology (IRCSET) postgraduate scholarship under the EMBARK initiative, and by the Science Foundation Ireland Research Frontiers Programme (SFI-RFP/ENM1726) and Short Term Travel Fellowship (SFI-STTF) | en_IE |
dc.format | application/pdf | en_IE |
dc.language.iso | en | en_IE |
dc.publisher | The Royal Society Publishing | en_IE |
dc.relation.ispartof | Journal Of The Royal Society Interface | en |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Ireland | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/3.0/ie/ | |
dc.subject | Cell mechanics | en_IE |
dc.subject | Actin cytoskeleton | en_IE |
dc.subject | Cell contractility | en_IE |
dc.subject | Chondrocyte | en_IE |
dc.subject | Finite element | en_IE |
dc.subject | In vitro shear | en_IE |
dc.subject | Gene expression | en_IE |
dc.subject | Micropipette aspiration | en_IE |
dc.subject | Chondrocyte cytoskeleton | en_IE |
dc.subject | Viscoelastic properties | en_IE |
dc.subject | Mechanical compression | en_IE |
dc.subject | Articular cartilage | en_IE |
dc.subject | Agarose constructs | en_IE |
dc.subject | Confocal analysis | en_IE |
dc.subject | In vitro | en_IE |
dc.subject | Nucleus | en_IE |
dc.subject | Mechanical engineering | en_IE |
dc.subject | Biomedical engineering | en_IE |
dc.title | The effect of remodelling and contractility of the actin cytoskeleton on the shear resistance of single cells: a computational and experimental investigation | en_IE |
dc.type | Article | en_IE |
dc.date.updated | 2016-07-29T15:04:39Z | |
dc.identifier.doi | 10.1098/rsif.2012.0428 | |
dc.local.publishedsource | http:/dx.doi.org/10.1098/rsif.2012.0428 | en_IE |
dc.description.peer-reviewed | peer-reviewed | |
dc.contributor.funder | |~| | |
dc.internal.rssid | 2885132 | |
dc.local.contact | William Ronan, Mechanical Engineering, School Of Engineering, Nui Galway. Email: william.ronan@nuigalway.ie | |
dc.local.copyrightchecked | No | |
dc.local.version | ACCEPTED | |
nui.item.downloads | 220 | |