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dc.contributor.authorDowling, Enda P.
dc.contributor.authorRonan, William
dc.contributor.authorOfek, Gidon
dc.contributor.authorDeshpande, Vikram S.
dc.contributor.authorMcMeeking, Robert M.
dc.contributor.authorAthanasiou, Kyriacos A.
dc.date.accessioned2016-08-10T07:47:21Z
dc.date.available2016-08-10T07:47:21Z
dc.date.issued2012-07-18
dc.identifier.citationDowling, Enda P., Ronan, William, Ofek, Gidon, Deshpande, Vikram S., McMeeking, Robert M., Athanasiou, Kyriacos A., & McGarry, J. Patrick. (2012). The effect of remodelling and contractility of the actin cytoskeleton on the shear resistance of single cells: a computational and experimental investigation. Journal of The Royal Society Interface, 9(77), 3469-3479. doi: 10.1098/rsif.2012.0428en_IE
dc.identifier.issn1742-5662
dc.identifier.urihttp://hdl.handle.net/10379/5939
dc.description.abstractThe biomechanisms that govern the response of chondrocytes to mechanical stimuli are poorly understood. In this study, a series of in vitro tests are performed, in which single chondrocytes are subjected to shear deformation by a horizontally moving probe. Dramatically different probe force-indentation curves are obtained for untreated cells and for cells in which the actin cytoskeleton has been disrupted. Untreated cells exhibit a rapid increase in force upon probe contact followed by yielding behaviour. Cells in which the contractile actin cytoskeleton was removed exhibit a linear force-indentation response. In order to investigate the mechanisms underlying this behaviour, a three-dimensional active modelling framework incorporating stress fibre (SF) remodelling and contractility is used to simulate the in vitro tests. Simulations reveal that the characteristic force-indentation curve observed for untreated chondrocytes occurs as a result of two factors: (i) yielding of SFs due to stretching of the cytoplasm near the probe and (ii) dissociation of SFs due to reduced cytoplasm tension at the front of the cell. In contrast, a passive hyperelastic model predicts a linear force-indentation curve similar to that observed for cells in which the actin cytoskeleton has been disrupted. This combined modelling-experimental study offers a novel insight into the role of the active contractility and remodelling of the actin cytoskeleton in the response of chondrocytes to mechanical loading.en_IE
dc.description.sponsorshipIrish Research Council for Science, Engineering and Technology (IRCSET) postgraduate scholarship under the EMBARK initiative, and by the Science Foundation Ireland Research Frontiers Programme (SFI-RFP/ENM1726) and Short Term Travel Fellowship (SFI-STTF)en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherThe Royal Society Publishingen_IE
dc.relation.ispartofJournal Of The Royal Society Interfaceen
dc.subjectCell mechanicsen_IE
dc.subjectActin cytoskeletonen_IE
dc.subjectCell contractilityen_IE
dc.subjectChondrocyteen_IE
dc.subjectFinite elementen_IE
dc.subjectIn vitro shearen_IE
dc.subjectGene expressionen_IE
dc.subjectMicropipette aspirationen_IE
dc.subjectChondrocyte cytoskeletonen_IE
dc.subjectViscoelastic propertiesen_IE
dc.subjectMechanical compressionen_IE
dc.subjectArticular cartilageen_IE
dc.subjectAgarose constructsen_IE
dc.subjectConfocal analysisen_IE
dc.subjectIn vitroen_IE
dc.subjectNucleusen_IE
dc.subjectMechanical engineeringen_IE
dc.subjectBiomedical engineeringen_IE
dc.titleThe effect of remodelling and contractility of the actin cytoskeleton on the shear resistance of single cells: a computational and experimental investigationen_IE
dc.typeArticleen_IE
dc.date.updated2016-07-29T15:04:39Z
dc.identifier.doi10.1098/rsif.2012.0428
dc.local.publishedsourcehttp:/dx.doi.org/10.1098/rsif.2012.0428en_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid2885132
dc.local.contactWilliam Ronan, Mechanical Engineering, School Of Engineering, Nui Galway. Email: william.ronan@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
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