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dc.contributor.authorRonan, William
dc.contributor.authorDeshpande, Vikram S.
dc.contributor.authorMcMeeking, Robert M.
dc.contributor.authorMcGarry, J. Patrick
dc.date.accessioned2016-08-09T14:39:15Z
dc.date.available2016-08-09T14:39:15Z
dc.date.issued2013-06-18
dc.identifier.citationRonan, William, Deshpande, Vikram S., McMeeking, Robert M., & McGarry, J. Patrick. (2014). Cellular contractility and substrate elasticity: a numerical investigation of the actin cytoskeleton and cell adhesion. Biomechanics and Modeling in Mechanobiology, 13(2), 417-435. doi: 10.1007/s10237-013-0506-zen_IE
dc.identifier.issn1617-7940
dc.identifier.urihttp://hdl.handle.net/10379/5937
dc.description.abstractNumerous experimental studies have established that cells can sense the stiffness of underlying substrates and have quantified the effect of substrate stiffness on stress fibre formation, focal adhesion area, cell traction, and cell shape. In order to capture such behaviour, the current study couples a mixed mode thermodynamic and mechanical framework that predicts focal adhesion formation and growth with a material model that predicts stress fibre formation, contractility, and dissociation in a fully 3D implementation. Simulations reveal that SF contractility plays a critical role in the substrate-dependent response of cells. Compliant substrates do not provide sufficient tension for stress fibre persistence, causing dissociation of stress fibres and lower focal adhesion formation. In contrast, cells on stiffer substrates are predicted to contain large amounts of dominant stress fibres. Different levels of cellular contractility representative of different cell phenotypes are found to alter the range of substrate stiffness that cause the most significant changes in stress fibre and focal adhesion formation. Furthermore, stress fibre and focal adhesion formation evolve as a cell spreads on a substrate and leading to the formation of bands of fibres leading from the cell periphery over the nucleus. Inhibiting the formation of FAs during cell spreading is found to limit stress fibre formation. The predictions of this mutually dependent material-interface framework are strongly supported by experimental observations of cells adhered to elastic substrates and offer insight into the inter-dependent biomechanical processes regulating stress fibre and focal adhesion formation.en_IE
dc.description.sponsorshipIrish Research Council for Science, Engineering and Technology (IRCSET) postgraduate scholarship under the EMBARK initiative and by the Science Foundation Ireland Research Frontiers Programme (SFI-RFP/ENM1726)en_IE
dc.formatapplication/pdfen_IE
dc.language.isoenen_IE
dc.publisherSpringer Verlagen_IE
dc.relation.ispartofBiomechanics And Modeling In Mechanobiologyen
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Ireland
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/ie/
dc.subjectStress fibre contractilityen_IE
dc.subjectFocal adhesion formationen_IE
dc.subjectSubstrate elasticityen_IE
dc.subjectNucleus stressen_IE
dc.subjectFinite elementen_IE
dc.subjectActive constitutive formulationen_IE
dc.subjectStress fiberen_IE
dc.subjectSmooth muscleen_IE
dc.subjectMechanical propertiesen_IE
dc.subjectMatrix elasticityen_IE
dc.subjectSignal transductionen_IE
dc.subjectFocal adhesionsen_IE
dc.subjectSingle cellsen_IE
dc.subjectStiffnessen_IE
dc.subjectModelen_IE
dc.subjectForceen_IE
dc.subjectMechanical engineeringen_IE
dc.titleCellular contractility and substrate elasticity: a numerical investigation of the actin cytoskeleton and cell adhesionen_IE
dc.typeArticleen_IE
dc.date.updated2016-07-29T14:59:08Z
dc.identifier.doi10.1007/s10237-013-0506-z
dc.local.publishedsourcehttp:/dx.doi.org/10.1007/s10237-013-0506-zen_IE
dc.description.peer-reviewedpeer-reviewed
dc.contributor.funder|~|
dc.internal.rssid6470111
dc.local.contactWilliam Ronan, Mechanical Engineering, School Of Engineering, Nui Galway. Email: william.ronan@nuigalway.ie
dc.local.copyrightcheckedNo
dc.local.versionACCEPTED
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Attribution-NonCommercial-NoDerivs 3.0 Ireland
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Ireland